Recombinant Human LAMA1 protein

中文名： 层粘连蛋白α1(LAMA1)重组蛋白
别名： LAMA1；LAMA；Laminin subunit alpha-1

货号: PA1000-8084

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	LAMA1
Uniprot No	P25391
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	全长
氨基酸序列	full
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与LAMA1重组蛋白相关的文献摘要概览：

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1. **文献名称**：*Recombinant expression and functional characterization of laminin α1 chain in epithelial morphogenesis*

**作者**：Miner JH et al.

**摘要**：研究通过昆虫细胞表达系统成功制备了全长LAMA1重组蛋白，验证其与整合素受体的结合活性，并证明其在体外可促进肾小管上皮细胞的三维结构形成。

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2. **文献名称**：*Structural analysis of the laminin α1 chain LG4-5 domain reveals heparin-binding motifs*

**作者**：Timpl R et al.

**摘要**：利用哺乳动物HEK293细胞表达LAMA1的LG4-5结构域重组蛋白，通过X射线晶体学解析其三维结构，发现该区域含有关键肝素结合位点，可能与组织再生相关。

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3. **文献名称**：*LAMA1 mutations disrupt basement membrane assembly and cause cerebellar dysplasia*

**作者**：Radner S et al.

**摘要**：研究通过重组LAMA1蛋白功能挽救实验，证明患者来源的LAMA1突变体无法形成稳定的基底膜结构，揭示了LAMA1在神经系统发育中的关键作用。

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*注：以上文献为示例性质，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"LAMA1 recombinant protein"+"expression/function/structure"进一步筛选近年研究。*

背景信息

LAMA1 recombinant protein is derived from the human LAMA1 gene, which encodes the alpha-1 subunit of laminin, a critical component of the extracellular matrix (ECM). Laminins are heterotrimeric glycoproteins composed of alpha, beta, and gamma chains, playing key roles in cell adhesion, differentiation, and tissue organization. The LAMA1 subunit specifically contributes to the formation of laminin-111 (α1β1γ1) and laminin-211 (α2β1γ1), which are essential for basement membrane integrity and cellular signaling in various tissues, including the nervous system, muscles, and kidneys.

Recombinant LAMA1 is produced using expression systems such as mammalian cells (e.g., HEK293 or CHO) or insect cells to ensure proper post-translational modifications, including glycosylation, which is crucial for its structural and functional properties. The purified protein typically retains domains like the laminin N-terminal (LN), laminin-type EGF-like (LEa), and globular (G) domains, enabling interactions with cell surface receptors (e.g., integrins) and ECM components.

Research applications of LAMA1 recombinant protein include studying cell-ECM interactions, tissue regeneration, and disease mechanisms. Mutations or deficiencies in LAMA1 are linked to disorders such as congenital muscular dystrophy, cerebellar dysplasia, and Pierson syndrome. By providing a controlled source of functional LAMA1. the recombinant protein aids in elucidating its role in development and pathology, as well as in developing therapeutic strategies for ECM-related diseases. Additionally, it serves as a vital tool in bioengineering scaffolds for regenerative medicine and drug screening platforms targeting ECM-associated pathways.