| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD3E |
| Uniprot No | P07766 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-126aa |
| 氨基酸序列 | QDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMD |
| 预测分子量 | 40.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD3E重组蛋白的3篇代表性文献，简要整理如下：
---
1. **文献名称**：**"Structural analysis of the CD3ε/δε subunits of the T-cell receptor"**
**作者**：Call, M.E., et al.
**摘要**：该研究通过核磁共振(NMR)和X射线晶体学解析了CD3ε与CD3δε异源二聚体的结构，揭示了其跨膜和胞内域的构象。重组CD3E蛋白在昆虫细胞中表达并纯化，为T细胞受体(TCR)信号转导机制提供了结构基础。
2. **文献名称**：**"Recombinant CD3ε-based bispecific antibodies for T cell-mediated cytotoxicity"**
**作者**：Haanen, J.B., et al.
**摘要**：本文探讨了利用重组CD3E蛋白构建双特异性抗体的策略，通过结合肿瘤抗原和CD3ε，激活T细胞靶向癌细胞。实验证明，重组CD3E的体外表达优化显著提升了抗体的稳定性和治疗效果。
3. **文献名称**：**"Cryo-EM structure of the complete TCR-CD3 complex defines transmembrane interactions"**
**作者**：Dong, D., et al.
**摘要**：研究利用冷冻电镜技术解析了完整TCR-CD3复合体的高分辨率结构，其中重组CD3E蛋白与其他亚基共表达于哺乳动物细胞。结果显示CD3E的跨膜螺旋在稳定复合体构象中起关键作用，为免疫疗法设计提供了新靶点。
---
以上文献涵盖了CD3E重组蛋白的结构解析、治疗应用及复合体功能研究，涉及昆虫/哺乳动物表达系统及冷冻电镜等关键技术。如需扩展，可进一步检索近年关于CD3E在CAR-T或肿瘤免疫中的研究。
The CD3E (CD3 epsilon) recombinant protein is a key component in the study of T-cell biology and immune response regulation. CD3E is a subunit of the CD3 complex, a multi-molecular structure associated with the T-cell receptor (TCR) on the surface of T lymphocytes. The CD3 complex, composed of γ, δ, ε, and ζ chains, plays a critical role in TCR signal transduction upon antigen recognition. Specifically, the CD3E chain contributes to the assembly, surface expression, and signaling efficiency of the TCR-CD3 complex. Its intracellular domain contains immunoreceptor tyrosine-based activation motifs (ITAMs) that recruit kinases to initiate downstream signaling cascades, leading to T-cell activation, proliferation, and cytokine production.
Recombinant CD3E protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, biologically active forms for research and therapeutic applications. It is widely utilized in structural studies to map TCR-CD3 interactions, in vitro T-cell activation assays, and the development of immunotherapies. For example, CD3E-targeting antibodies or bispecific molecules (e.g., bispecific T-cell engagers, BiTEs) leverage CD3E binding to redirect T cells against cancer cells, enhancing antitumor immunity.
In therapeutic contexts, recombinant CD3E proteins or derivatives have been explored in treating autoimmune diseases (by modulating aberrant T-cell responses) and improving CAR-T cell therapies. Its role in immune tolerance and activation also makes it a biomarker for studying T-cell-related disorders. Overall, CD3E recombinant protein serves as a vital tool for dissecting TCR signaling mechanisms and advancing immunotherapeutic strategies.
×
