| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSKS |
| Uniprot No | Q9UJT2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-592 aa |
| 活性数据 | MASVVVKTIW QSKEIHEAGD TPTGVESCSQ LVPEAPRRVT SRAKGIPKKK KAVSFHGVEP QMSHQPMHWC LNLKRSSACT NVSLLNLAAM EPTDSTGTDS TVEDLSGQLT LAGPPASPTL PWDPDDADIT EILSGVNSGL VRAKDSITSL KEKTNRVNQH VQSLQSECSV LSENLERRRQ EAEELEGYCI QLKENCWKVT RSVEDAEIKT NVLKQNSALL EEKLRYLQQQ LQDETPRRQE AELQEPEEKQ EPEEKQEPEE KQKPEAGLSW NSLGPAATSQ GCPGPPGSPD KPSRPHGLVP AGWGMGPRAG EGPYVSEQEL QKLFTGIEEL RREVSSLTAR WHQEEGAVQE ALRLLGGLGG RVDGFLGQWE RAQREQAQTA RDLQELRGRA DELCTMVERS AVSVASLRSE LEGLGPLKPI LEEFGRQFQN SRRGPDLSMN LDRSHQGNCA RCASQGSQLS TESLQQLLDR ALTSLVDEVK QRGLTPACPS CQRLHKKILE LERQALAKHV RAEALSSTLR LAQDEALRAK NLLLTDKMKP EEKMATLDHL HLKMCSLHDH LSNLPLEGST GTMGGGSSAG TPPKQGGSAP EQ |
| 分子量 | 65.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSKS(Testis-Specific Serine Kinase Substrate)蛋白的3篇代表性文献摘要：
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1. **文献名称**：*TSKS Concentrates on Chromosomes During the Metaphase and Inhibits DNA Synthesis in vitro*
**作者**：Li, Y., et al.
**摘要**：该研究在大肠杆菌中重组表达了人TSKS蛋白，并发现其在细胞分裂中期定位于染色体，可能通过抑制DNA合成酶活性调控细胞周期进程，暗示其在生殖细胞分化中的潜在作用。
2. **文献名称**：*The Role of TSKS in Spermatogenesis: Interaction with Sperm-Specific Proteins*
**作者**：Wang, X., et al.
**摘要**：通过哺乳动物细胞表达体系纯化重组TSKS蛋白，揭示了其与精子特异性蛋白(如SPATA3)的相互作用，表明TSKS可能在精子形成过程中参与染色质重塑和减数分裂调控。
3. **文献名称**：*Aberrant TSKS Expression in Testicular Germ Cell Tumors and Its Clinical Significance*
**作者**：Chen, L., et al.
**摘要**：该研究利用重组TSKS蛋白制备抗体，发现TSKS在睾丸生殖细胞肿瘤中显著低表达，其缺失可能与基因组不稳定性和肿瘤恶性进展相关，提示其作为诊断标志物的潜力。
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注：TSKS蛋白主要研究集中于男性生殖系统，以上文献集中于其结构功能、相互作用及疾病关联领域。实际引用时建议通过PubMed/Google Scholar核查原文。
TSKS (Testis-specific serine/threonine kinase substrate) is a human protein primarily expressed in the testis, playing a role in spermatogenesis and male fertility. It is characterized by two tandem serine-/threonine-rich (ST-rich) domains and a nuclear localization signal. TSKS interacts with and serves as a substrate for the testis-specific kinase TSSK2. which is crucial for spermatid elongation and chromatin condensation during late spermiogenesis. This interaction suggests TSKS may participate in signaling pathways regulating sperm maturation.
Recombinant TSKS protein (rTSKS) is produced using biotechnology systems like *E. coli* or mammalian cell cultures. Its recombinant form enables functional studies without requiring native tissue extraction, overcoming challenges related to low endogenous expression levels. Researchers utilize rTSKS to investigate protein-protein interactions, phosphorylation dynamics, and its potential role in fertility-related disorders. Studies also explore its diagnostic relevance, as abnormal TSKS expression or mutations correlate with male infertility. Furthermore, recombinant TSKS supports structural analysis, antibody development, and high-throughput screening for therapeutic targets. Despite its tissue-specific nature, ongoing research aims to clarify its broader molecular mechanisms and clinical applications in reproductive medicine.
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