纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | TSGA10 |
Uniprot No | Q3SY00 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-556 aa |
活性数据 | MGQDTDMLNT YQQLVRTPSV RPGQDVRLQA PGTRTGLLKL LSTVSQDKQG CLGSGDGVPN QDLQQRSQSS RQTAKKDRKP RGQSKKGQGS EESEDHFPLL PRKPSFPFQW AWESIATDVR AVLQPSSPTP GHQALPMPSS FSQRQSRRKS TANLPEAHGC CWKTEAQNLK ARQQLGAWGG VSIPTGKGEL GSEPPSGLQL PGRRPGSGSA SDKQVQLQSL GAEEAERGLS SGVLPQRPRR GSISEEEQFS EATEEAEEGE HRTPCRRRAG CQRKGQISGE EASDEGEVQG QSQGSSPSFN NLRRRQWRKT RAKELQGPWD LEKLHRQLQR DLDCGPQKLP WKTLRAAFQA SKRNGKAYAS GYDETFVSAN LPNRTFHKRQ EATRSLLQAW ERQRQEERQQ AELRRARTQH VQRQVAHCLA AYAPRGSRGP GAAQRKLEEL RRQERQRFAE YQAELQGIQH RVQARPFLFQ QAMQANARLT VTRRFSQVLS ALGLDEEQLL SEAGKVDREG TPRKPRSHRS VGVRMEHSPQ RPPRTEPTGS QPDRHYNPSL DPECSP |
分子量 | 62.3 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSGA10蛋白的3-4篇参考文献(示例虚构,供参考):
1. **文献名称**: *TSGA10 inhibits HIF1α nuclear translocation in breast cancer*
**作者**: Modarressi, M.H. et al.
**摘要**: 本研究揭示TSGA10通过干扰HIF1α的核转运抑制乳腺癌细胞血管生成,其表达缺失与肿瘤恶性进展相关。
2. **文献名称**: *Role of TSGA10 in sperm head-tail coupling*
**作者**: Bahreinian, M. et al.
**摘要**: 发现TSGA10蛋白在小鼠精子尾部形成中调控结构蛋白组装,其突变导致精子头部-尾部连接异常和不育。
3. **文献名称**: *TSGA10 as a biomarker in prostate cancer*
**作者**: Chen, L. et al.
**摘要**: 临床研究显示前列腺癌中TSGA10表达显著下调,其低表达与肿瘤转移和患者生存率降低相关。
4. **文献名称**: *TSGA10 interactions with centrosomal proteins*
**作者**: Khoshghamat, S. et al.
**摘要**: 通过蛋白质组学分析,TSGA10被发现与中心体蛋白CEP135相互作用,提示其在细胞分裂中的潜在调控功能。
注:以上文献及内容为示例,实际研究中需查询具体发表的论文。建议通过PubMed/Google Scholar以“TSGA10 protein”为关键词获取真实文献。
**Background of Recombinant Human TSGA10 Protein**
Testis-Specific Gene Antigen 10 (TSGA10) is a protein encoded by the *TSGA10* gene, primarily expressed in the testis but also detected in other tissues, including the brain and certain cancer cells. It plays a critical role in spermatogenesis, cellular division, and cilia-related processes. Structurally, TSGA10 contains coiled-coil domains and a conserved DUF4218 motif, suggesting involvement in protein-protein interactions and microtubule organization. Its localization to the cytoplasm and basal body of cilia underscores its function in maintaining structural integrity during cell division and ciliogenesis.
Dysregulation of TSGA10 has been linked to male infertility, as mutations or altered expression disrupt sperm development. Additionally, TSGA10 is implicated in cancer progression, acting as a tumor suppressor in some contexts (e.g., inhibiting hypoxia-inducible factor 1α in breast cancer) while promoting aggressiveness in others (e.g., prostate cancer). Its dual roles highlight tissue-specific regulatory mechanisms.
Recombinant human TSGA10 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, interactions, and therapeutic potential. This tool aids in elucidating TSGA10's roles in fertility, cancer, and ciliopathies, offering avenues for diagnostics and targeted therapies.
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