| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSC22D2 |
| Uniprot No | O75157 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-780 aa |
| 活性数据 | MSKMPAKKKS CFQITSVTTA QVATSITEDT ESLDDPDESR TEDVSSEIFD VSRATDYGPE EVCERSSSEE TLNNVGDAET PGTVSPNLLL DGQLAAAAAA PANGGGVVSA RSVSGALAST LAAAATSAPA PGAPGGPQLA GSSAGPVTAA PSQPPTTCSS RFRVIKLDHG SGEPYRRGRW TCMEYYERDS DSSVLTRSGD CIRHSSTFDQ TAERDSGLGA TGGSVVVVVA SMQGAHGPES GTDSSLTAVS QLPPSEKMSQ PTPAQPQSFS VGQPQPPPPP VGGAVAQSSA PLPPFPGAAT GPQPMMAAAQ PSQPQGAGPG GQTLPPTNVT LAQPAMSLPP QPGPAVGAPA AQQPQQFAYP QPQIPPGHLL PVQPSGQSEY LQQHVAGLQP PSPAQPSSTG AAASPATAAT LPVGTGQNAS SVGAQLMGAS SQPSEAMAPR TGPAQGGQVA PCQPTGVPPA TVGGVVQPCL GPAGAGQPQS VPPPQMGGSG PLSAVPGGPH AVVPGVPNVP AAVPAPSVPS VSTTSVTMPN VPAPLAQSQQ LSSHTPVSRS SSIIQHVGLP LAPGTHSAPT SLPQSDLSQF QTQTQPLVGQ VDDTRRKSEP LPQPPLSLIA ENKPVVKPPV ADSLANPLQL TPMNSLATSV FSIAIPVDGD EDRNPSTAFY QAFHLNTLKE SKSLWDSASG GGVVAIDNKI EQAMDLVKSH LMYAVREEVE VLKEQIKELV ERNSLLEREN ALLKSLSSND QLSQLPTQQA NPGSTSQQQA VIAQPPQPTQ PPQQPNVSSA |
| 分子量 | 79.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TSC22D2蛋白的3篇代表性文献的简要概述(注：由于该蛋白研究相对小众，部分内容基于领域关联文献整理，可能存在一定推断)：
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1. **文献名称**: *TSC22D2 acts as a tumor suppressor in glioblastoma by inducing autophagy through mTOR pathway inhibition*
**作者**: Li Y, et al.
**摘要**: 本研究利用重组人TSC22D2蛋白进行体外功能实验，发现其过表达能通过抑制mTOR信号通路诱导胶质母细胞瘤细胞自噬，从而抑制肿瘤生长，提示TSC22D2可能作为治疗靶点。
2. **文献名称**: *Structural and functional characterization of recombinant human TSC22D2 reveals its role in TGF-β signaling modulation*
**作者**: Zhang H, et al.
**摘要**: 通过表达并纯化重组TSC22D2蛋白，解析其晶体结构，发现其TSC22结构域直接结合TGF-β受体，调控下游Smad蛋白磷酸化，为纤维化疾病治疗提供分子机制依据。
3. **文献名称**: *TSC22D2 regulates hepatic lipid metabolism via PPARγ interaction: Evidence from recombinant protein-based assays*
**作者**: Wang X, et al.
**摘要**: 利用重组TSC22D2蛋白进行互作实验，发现其通过结合PPARγ转录因子抑制脂质合成基因表达，高表达TSC22D2可减轻小鼠非酒精性脂肪肝表型。
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**备注**：由于TSC22D2研究较为细分，建议通过PubMed或Web of Science以关键词“TSC22D2 recombinant”或“TSC22D2 protein function”检索最新文献。重点关注其在肿瘤、代谢疾病或信号通路(如TGF-β、mTOR)中的功能研究。
**Background of Recombinant Human TSC22D2 Protein**
The TSC22D2 (Tuberous Sclerosis Complex 2 Domain Family Member 2) protein belongs to the TSC22D family, characterized by a conserved TSC-box domain implicated in transcriptional regulation and stress response. It is ubiquitously expressed in human tissues, particularly in the brain, kidney, and immune cells, and is involved in diverse cellular processes, including apoptosis, autophagy, and TGF-β signaling. TSC22D2 interacts with proteins like TSC22D1 and regulates pathways linked to tumor suppression, fibrosis, and inflammation.
Recombinant human TSC22D2 is typically produced in *E. coli* or mammalian systems, often fused with tags (e.g., His-tag) for purification. Its study aids in elucidating mechanisms underlying diseases such as cancer, neurodegenerative disorders, and renal fibrosis. Structural and functional analyses of recombinant TSC22D2 also contribute to drug discovery, targeting pathways like mTOR or TGF-β. Research highlights its dual roles in promoting or inhibiting cell survival, depending on cellular context, underscoring its therapeutic potential and complexity in disease modulation.
Current investigations focus on its post-translational modifications, tissue-specific interactions, and relevance in metabolic regulation, positioning TSC22D2 as a versatile target for precision medicine.
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