| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXC1 |
| Uniprot No | Q12948 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MQARYSVSSPNSLGVVPYLGGEQSYYRAAAAAAGGGYTAMPAPMSVYSHPAHAEQYPGGMARAYGPYTPQPQPKDMVKPPYSYIALITMAIQNAPDKKITLNGIYQFIMDRFPFYRDNKQGWQNSIRHNLSLNECFVKVPRDDKKPGKGSYWTLDPDSYNMFENGSFLRRRRRFKKKDAVKDKEEKDRLHLKEPPPPGRQPPPAPPEQADGNAPGPQPPPVRIQDIKTENGTCPSPPQPLSPAAALGSGSAAAVPKIESPDSSSSSLSSGSSPPGSLPSARPLSLDGADSAPPPPAPSAPPPHHSQGFSVDNIMTSLRGSPQSAAAELSSGLLASAAASSRAGIAPPLALGAYSPGQSSLYSSPCSQTSSAGSSGGGGGGAGAAGGAGGAGTYHCNLQAMSLYAAGERGGHLQGAPGGAGGSAVDDPLPDYSLPPVTSSSSSSLSHGGGGGGGGGGQEAGHHPAAHQGRLTSWYLNQAGGDLGHLASAAAAAAAAGYPGQQQNFHSVREMFESQRIGLNNSPVNGNSSCQMAFPSSQSLYRTSGAFVYDCSKF |
| 预测分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXC1重组蛋白的模拟参考文献示例(内容为虚构，仅作格式参考)：
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1. **"Purification and functional analysis of recombinant FOXC1 transcription factor"**
*Smith A, et al. (2018)*
摘要：本研究描述了FOXC1重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其体外结合特定DNA序列的能力，为后续转录调控机制研究提供工具。
2. **"FOXC1 recombinant protein rescues cellular migration defects in corneal dystrophy models"**
*Lee J, et al. (2020)*
摘要：通过体外递送FOXC1重组蛋白至角膜细胞，恢复了因FOXC1突变导致的细胞迁移功能障碍，提示其在遗传性角膜疾病治疗中的潜在应用。
3. **"Structural insights into FOXC1-DNA interaction via crystallography of recombinant protein complexes"**
*Zhang Y, et al. (2021)*
摘要：解析了FOXC1重组蛋白与靶DNA的复合物晶体结构，揭示了关键氨基酸残基在识别DNA基序中的作用，为突变致病机制提供结构生物学依据。
4. **"Recombinant FOXC1 inhibits epithelial-mesenchymal transition in breast cancer cells"**
*Chen R, et al. (2019)*
摘要：证明外源性FOXC1重组蛋白可下调TGF-β信号通路，抑制乳腺癌细胞EMT进程，提示其作为肿瘤转移干预策略的可行性。
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注：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。
FOXC1. a member of the forkhead box (FOX) transcription factor family, plays critical roles in embryonic development, particularly in the formation of the anterior eye segment, cardiovascular system, and neural crest-derived tissues. It regulates gene expression by binding to DNA through its conserved forkhead domain, influencing cellular processes like proliferation, differentiation, and migration. Mutations in FOXC1 are linked to congenital disorders such as Axenfeld-Rieger syndrome, glaucoma, and heart defects, highlighting its biological and clinical significance.
Recombinant FOXC1 protein is engineered using genetic cloning techniques, where the FOXC1 gene is expressed in host systems (e.g., E. coli or mammalian cells) to produce purified, functional protein. This recombinant form retains the DNA-binding and regulatory properties of native FOXC1. enabling researchers to study its molecular interactions, signaling pathways, and downstream targets in controlled settings. It is often tagged (e.g., His-tag) for ease of purification and detection.
In research, recombinant FOXC1 serves as a tool to investigate disease mechanisms, particularly in developmental anomalies and ocular pathologies. For example, studies use it to analyze how FOXC1 dysfunction disrupts tissue morphogenesis or contributes to glaucoma pathogenesis. It also aids in screening potential therapeutic compounds targeting FOXC1-related pathways. Additionally, the protein is utilized in structural studies to map functional domains or mutation effects, providing insights into genotype-phenotype correlations.
Beyond basic research, recombinant FOXC1 holds potential in gene therapy and regenerative medicine, such as restoring normal function in cells with FOXC1 deficiencies. However, challenges remain in optimizing delivery methods and ensuring specificity in clinical applications. Overall, recombinant FOXC1 is a vital resource for unraveling the protein’s role in health and disease, bridging molecular biology with translational medicine.
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