| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRUB1 |
| Uniprot No | Q8WWH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-349 aa |
| 活性数据 | AASEAAVVS SPSLKTDTSP VLETAGTVAA MAATPSARAA AAVVAAAART GSEARVSKAA LATKLLSLSG VFAVHKPKGP TSAELLNRLK EKLLAEAGMP SPEWTKRKKQ TLKIGHGGTL DSAARGVLVV GIGSGTKMLT SMLSGSKRYT AIGELGKATD TLDSTGRVTE EKPYDKITQE DIEGILQKFT GNIMQVPPLY SALKKDGQRL STLMKRGEVV EAKPARPVTV YSISLQKFQP PFFTLDVECG GGFYIRSLVS DIGKELSSCA NVLELTRTKQ GPFTLEEHAL PEDKWTIDDI AQSLEHCSSL FPAELALKKS KPESNEQVLS CEYITLNEPK REDDVIKTC |
| 分子量 | 37.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRUB1蛋白的3篇代表性文献的简要列举(文献信息及摘要为示例性描述，可能不指向真实存在的论文)：
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1. **文献名称**: "TRUB1-mediated pseudouridylation of tRNA is essential for mammalian development"
**作者**: Smith A, et al.
**摘要**: 研究揭示了TRUB1作为哺乳动物中关键假尿苷合成酶，在tRNA特定位点修饰中的作用，并证明其缺失导致胚胎发育异常及细胞翻译功能受损。
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2. **文献名称**: "Structural and functional analysis of human TRUB1 in RNA modification"
**作者**: Zhang Y, et al.
**摘要**: 通过晶体结构解析，阐明了TRUB1催化RNA假尿苷化的分子机制，并发现其活性位点突变会导致RNA代谢紊乱及疾病相关通路失调。
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3. **文献名称**: "TRUB1 mutations linked to myelodysplastic syndrome disrupt tRNA pseudouridylation"
**作者**: Jones R, et al.
**摘要**: 该研究将TRUB1基因突变与骨髓增生异常综合征相关联，指出突变通过干扰tRNA修饰和蛋白质翻译，促进造血干细胞功能障碍。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“TRUB1 pseudouridine”获取最新研究。
TRUB1 (tRNA pseudouridine synthase 1) is a conserved eukaryotic enzyme belonging to the pseudouridine synthase family. It catalyzes the isomerization of uridine to pseudouridine (Ψ) at specific positions in transfer RNAs (tRNAs), a critical RNA modification that enhances tRNA stability, structural integrity, and translational fidelity. TRUB1 is particularly involved in Ψ formation at position 55 (Ψ55) in the T-loop of most tRNAs, a universally conserved modification implicated in ribosome binding and codon-anticodon interactions.
Localized predominantly in the nucleolus, TRUB1 interacts with other RNA-processing machinery, linking its function to broader RNA metabolism and ribosome biogenesis. Dysregulation of TRUB1 has been associated with human diseases, including myelodysplastic syndromes (MDS) and certain cancers, where mutations or altered expression may disrupt RNA modification patterns, leading to translational errors or impaired cellular homeostasis. Studies also suggest roles in viral infection responses and stress granule dynamics, highlighting its versatility. Structurally, TRUB1 contains a catalytic TruB domain conserved across species and a unique C-terminal extension that may regulate substrate recognition. While its precise regulatory mechanisms remain under investigation, TRUB1 exemplifies the functional significance of RNA modifications in cellular health and disease, making it a potential target for therapeutic exploration.
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