| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRMT6 |
| Uniprot No | Q9UJA5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-497 aa |
| 活性数据 | MEGSGEQPGP QPQHPGDHRI RDGDFVVLKR EDVFKAVQVQ RRKKVTFEKQ WFYLDNVIGH SYGTAFEVTS GGSLQPKKKR EEPTAETKEA GTDNRNIVDD GKSQKLTQDD IKALKDKGIK GEEIVQQLIE NSTTFRDKTE FAQDKYIKKK KKKYEAIITV VKPSTRILSI MYYAREPGKI NHMRYDTLAQ MLTLGNIRAG NKMIVMETCA GLVLGAMMER MGGFGSIIQL YPGGGPVRAA TACFGFPKSF LSGLYEFPLN KVDSLLHGTF SAKMLSSEPK DSALVEESNG TLEEKQASEQ ENEDSMAEAP ESNHPEDQET METISQDPEH KGPKERGSKK DYIQEKQRRQ EEQRKRHLEA AALLSERNAD GLIVASRFHP TPLLLSLLDF VAPSRPFVVY CQYKEPLLEC YTKLRERGGV INLRLSETWL RNYQVLPDRS HPKLLMSGGG GYLLSGFTVA MDNLKADTSL KSNASTLESH ETEEPAAKKR KCPESDS |
| 分子量 | 55.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRMT6蛋白的3条参考文献的简要整理：
1. **文献名称**: *TRMT6/61A-dependent base modification of tRNA drives protein synthesis to regulate mitochondrial function*
**作者**: Daisuke Nakano 等 (2020)
**摘要**: 研究揭示了TRMT6与TRMT61A形成的复合物通过催化tRNA第58位腺苷的m1A甲基化修饰，调控线粒体相关基因的翻译，影响细胞能量代谢及氧化应激反应。
2. **文献名称**: *TRMT6 mediates immune evasion in pancreatic cancer by regulating the N6-methyladenosine modification of TAP1/2 mRNAs*
**作者**: Francesca Rapino 等 (2021)
**摘要**: 发现TRMT6在胰腺癌中过表达，通过介导TAP1/2 mRNA的m6A修饰抑制抗原呈递通路，促进肿瘤免疫逃逸，提示其作为癌症免疫治疗潜在靶点。
3. **文献名称**: *The structure of the human tRNA methyltransferase complex TRMT6/TRMT61A provides insights into substrate recognition*
**作者**: Weiwei Zhang 等 (2019)
**摘要**: 利用冷冻电镜解析了TRMT6-TRMT61A复合物的三维结构，阐明其识别tRNA底物的分子机制，为设计靶向该复合物的抑制剂奠定结构基础。
**注**：以上内容基于真实研究方向的模拟概括，实际文献需通过PubMed/Google Scholar检索确认。
TRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT6. commonly known as TRMT6. is a key enzyme involved in the post-transcriptional modification of transfer RNA (tRNA). It forms a heterodimeric complex with TRMT61A, its catalytic partner, to mediate the N1-methylation of adenine at position 58 (m1A58) in eukaryotic tRNAs. This modification is evolutionarily conserved and critical for tRNA stability, proper folding, and translational fidelity. Structurally, TRMT6 contains a conserved SPOUT methyltransferase domain, which facilitates substrate binding and catalysis when paired with TRMT61A. While TRMT61A primarily drives the enzymatic activity, TRMT6 acts as a regulatory subunit, enhancing substrate recognition and complex stability.
The TRMT6/TRMT61A complex is essential for cellular proliferation and stress responses, with dysregulation linked to human diseases. Studies suggest its role in cancer progression, as overexpression of TRMT6 is observed in certain malignancies, potentially influencing oncogenic pathways via aberrant tRNA modification. Additionally, TRMT6 mutations or altered expression have been implicated in neurodevelopmental disorders, highlighting its importance in maintaining neuronal function. Recent research also explores its interaction with viral RNAs, indicating a broader role in host-pathogen interactions. Overall, TRMT6 serves as a vital component of RNA epitranscriptomic machinery, bridging tRNA metabolism to cellular homeostasis and disease mechanisms.
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