| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRMT2A |
| Uniprot No | Q8IZ69 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-625 aa |
| 活性数据 | MSENLDNEGP KPMESCGQES SSALSCPTVS VPPAAPAALE EVEKEGAGAA TGPGPQPGLY SYIRDDLFTS EIFKLELQNV PRHASFSDVR RFLGRFGLQP HKTKLFGQPP CAFVTFRSAA ERDKALRVLH GALWKGRPLS VRLARPKADP MARRRRQEGE SEPPVTRVAD VVTPLWTVPY AEQLERKQLE CEQVLQKLAK EIGSTNRALL PWLLEQRHKH NKACCPLEGV RPSPQQTEYR NKCEFLVGVG VDGEDNTVGC RLGKYKGGTC AVAAPFDTVH IPEATKQVVK AFQEFIRSTP YSAYDPETYT GHWKQLTVRT SRRHQAMAIA YFHPQKLSPE ELAELKTSLA QHFTAGPGRA SGVTCLYFVE EGQRKTPSQE GLPLEHVAGD RCIHEDLLGL TFRISPHAFF QVNTPAAEVL YTVIQDWAQL DAGSMVLDVC CGTGTIGLAL ARKVKRVIGV ELCPEAVEDA RVNAQDNELS NVEFHCGRAE DLVPTLVSRL ASQHLVAILD PPRAGLHSKV ILAIRRAKNL RRLLYVSCNP RAAMGNFVDL CRAPSNRVKG IPFRPVKAVA VDLFPQTPHC EMLILFERVE HPNGTGVLGP HSPPAQPTPG PPDNTLQETG TFPSS |
| 分子量 | 68.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人TRMT2A蛋白相关的研究文献摘要概括(基于公开信息模拟，非真实文献)：
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1. **"Structural and functional characterization of human TRMT2A reveals its tRNA methyltransferase activity"**
*作者：Li X. et al. (2020)*
**摘要**：本研究解析了重组人TRMT2A蛋白的晶体结构，证实其通过SAM依赖性催化tRNA的第5位胞嘧啶(m5C)甲基化，并揭示了特定结构域对底物识别的关键作用。
2. **"TRMT2A links tRNA modification to oxidative stress response in mammalian cells"**
*作者：Park S. & Kim J.H. (2018)*
**摘要**：利用重组TRMT2A蛋白进行体外实验，发现其介导的tRNA修饰参与调节细胞氧化应激反应，缺失TRMT2A会导致线粒体功能异常及活性氧(ROS)累积。
3. **"Dysregulation of TRMT2A in breast cancer promotes tumor progression via rRNA biogenesis"**
*作者：Chen L. et al. (2021)*
**摘要**：研究通过重组TRMT2A过表达模型，发现其异常表达通过影响核糖体RNA加工激活mTOR通路，促进乳腺癌细胞增殖和转移。
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*注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar等数据库检索确认。*
**Background of Human TRMT2A Protein**
TRMT2A (tRNA Methyltransferase 2 Homolog A) is a conserved eukaryotic protein primarily known for its role in post-transcriptional RNA modification. As a member of the methyltransferase superfamily, it catalyzes the transfer of methyl groups to specific nucleotides in tRNA molecules. TRMT2A is implicated in the formation of N2.N2-dimethylguanosine (m²₂G) at position 6 in certain tRNAs, a modification critical for maintaining tRNA stability, structure, and accurate translation. Structurally, TRMT2A contains a conserved SAM-dependent methyltransferase domain and a zinc finger motif, which may assist in RNA binding or protein interactions.
Beyond tRNA methylation, TRMT2A has been linked to diverse cellular processes, including stress response, cell cycle regulation, and DNA repair. Studies suggest it interacts with proteins involved in RNA processing and may influence pathways like p53 signaling. Dysregulation of TRMT2A has been associated with human diseases, such as cancers and neurological disorders, though its precise mechanistic roles remain under investigation.
Recombinant human TRMT2A protein, often expressed in *E. coli* or mammalian systems, is utilized to study its enzymatic activity, structure-function relationships, and molecular interactions *in vitro*. Its conserved nature across species highlights its fundamental biological importance, making it a target for exploring RNA modification-related therapeutics. Further research aims to clarify its regulatory networks and potential as a biomarker or drug target.
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