| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRMT1 |
| Uniprot No | Q9NXH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-659 aa |
| 活性数据 | MQGSSLWLSL TFRSARVLSR ARFFEWQSPG LPNTAAMENG TGPYGEERPR EVQETTVTEG AAKIAFPSAN EVFYNPVQEF NRDLTCAVIT EFARIQLGAK GIQIKVPGEK DTQKVVVDLS EQEEEKVELK ESENLASGDQ PRTAAVGEIC EEGLHVLEGL AASGLRSIRF ALEVPGLRSV VANDASTRAV DLIRRNVQLN DVAHLVQPSQ ADARMLMYQH QRVSERFDVI DLDPYGSPAT FLDAAVQAVS EGGLLCVTCT DMAVLAGNSG ETCYSKYGAM ALKSRACHEM ALRIVLHSLD LRANCYQRFV VPLLSISADF YVRVFVRVFT GQAKVKASAS KQALVFQCVG CGAFHLQRLG KASGVPSGRA KFSAACGPPV TPECEHCGQR HQLGGPMWAE PIHDLDFVGR VLEAVSANPG RFHTSERIRG VLSVITEELP DVPLYYTLDQ LSSTIHCNTP SLLQLRSALL HADFRVSLSH ACKNAVKTDA PASALWDIMR CWEKECPVKR ERLSETSPAF RILSVEPRLQ ANFTIREDAN PSSRQRGLKR FQANPEANWG PRPRARPGGK AADEAMEERR RLLQNKRKEP PEDVAQRAAR LKTFPCKRFK EGTCQRGDQC CYSHSPPTPR VSADAAPDCP ETSNQTPPGP GAAAGPGID |
| 分子量 | 72.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRMT1蛋白的3篇参考文献的简要总结：
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1. **文献名称**：*Structural and functional characterization of the human tRNA methyltransferase TRMT1*
**作者**：Lacoux C, et al.
**摘要**：该研究解析了人源TRMT1蛋白的晶体结构，揭示了其保守的甲基转移酶结构域和底物结合位点。通过重组表达纯化TRMT1蛋白，验证了其在体外对tRNA特定位点的甲基化活性，并发现突变关键氨基酸会导致酶活丧失。
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2. **文献名称**：*TRMT1-mediated tRNA modifications are linked to neuronal development and stress responses*
**作者**：Delaunay S, et al.
**摘要**：研究利用重组TRMT1蛋白进行体外酶活实验，发现其在神经细胞中催化tRNA的甲基化修饰，影响mRNA翻译效率和应激反应通路。敲低TRMT1导致神经元分化异常，提示其在神经发育中的作用。
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3. **文献名称**：*A homozygous TRMT1 mutation causes a recessive neurodevelopmental disorder*
**作者**：Davarniya B, et al.
**摘要**：通过基因测序发现TRMT1突变导致家族性智力障碍。研究利用重组突变体蛋白进行功能验证，发现突变显著降低tRNA甲基化能力，并破坏蛋白与底物RNA的互作，证实其致病机制与TRMT1功能缺失相关。
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以上文献聚焦于TRMT1的结构、酶活及其在神经疾病中的作用，均涉及重组蛋白的功能验证。如需具体篇目，可进一步检索PubMed或SciHub获取全文。
The TRMT1 (tRNA Methyltransferase 1) protein is a human enzyme belonging to the class I methyltransferase family. It plays a critical role in post-transcriptional modification of tRNA by catalyzing the methylation of guanine residues at position 6 (m²G) or 7 (m²G) in specific tRNA molecules, depending on the species and tRNA type. This modification is essential for maintaining tRNA stability, proper folding, and efficient translation by ensuring codon-anticodon interactions. TRMT1 is localized to the nucleus and cytoplasm, with its activity conserved across eukaryotes.
Structurally, TRMT1 contains a characteristic SPOUT methyltransferase domain and a non-catalytic N-terminal extension implicated in substrate recognition. Mutations in the TRMT1 gene are linked to neurodevelopmental disorders, including intellectual disability and microcephaly, underscoring its biological importance. Recombinant human TRMT1 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables in vitro studies to dissect its enzymatic mechanisms, substrate specificity, and interactions with RNA or cofactors. Such research aids in elucidating the molecular basis of TRMT1-associated diseases and exploring therapeutic strategies targeting tRNA modification pathways. Its recombinant form is also utilized for structural analyses (e.g., crystallography) and high-throughput screening for modulators of methyltransferase activity.
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