| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXO1 |
| Uniprot No | Q12778 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-655aa |
| 氨基酸序列 | MAEAPQVVEI DPDFEPLPRP RSCTWPLPRP EFSQSNSATS SPAPSGSAAA NPDAAAGLPS ASAAAVSADF MSNLSLLEES EDFPQAPGSV AAAVAAAAAA AATGGLCGDF QGPEAGCLHP APPQPPPPGP LSQHPPVPPA AAGPLAGQPR KSSSSRRNAW GNLSYADLIT KAIESSAEKR LTLSQIYEWM VKSVPYFKDK GDSNSSAGWK NSIRHNLSLH SKFIRVQNEG TGKSSWWMLN PEGGKSGKSP RRRAASMDNN SKFAKSRSRA AKKKASLQSG QEGAGDSPGS QFSKWPASPG SHSNDDFDNW STFRPRTSSN ASTISGRLSP IMTEQDDLGE GDVHSMVYPP SAAKMASTLP SLSEISNPEN MENLLDNLNL LSSPTSLTVS TQSSPGTMMQ QTPCYSFAPP NTSLNSPSPN YQKYTYGQSS MSPLPQMPIQ TLQDNKSSYG GMSQYNCAPG LLKELLTSDS PPHNDIMTPV DPGVAQPNSR VLGQNVMMGP NSVMSTYGSQ ASHNKMMNPS SHTHPGHAQQ TSAVNGRPLP HTVSTMPHTS GMNRLTQVKT PVQVPLPHPM QMSALGGYSS VSSCNGYGRM GLLHQEKLPS DLDGMFIERL DCDMESIIRN DLMDGDTLDF NFDNVLPNQS FPHSVKTTTH SWVSG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FOXO1重组蛋白的代表性文献(虚构示例，实际引用请核查原文)：
1. **《FOXO1重组蛋白的晶体结构揭示其DNA结合机制》**
*作者：Zhang Y. et al. (2020)*
摘要：通过X射线晶体学解析了人源FOXO1重组蛋白的DNA结合域三维结构，揭示了其与靶基因启动子特异性结合的分子机制，为设计FOXO1调控药物提供结构基础。
2. **《重组FOXO1蛋白在肝癌细胞凋亡中的功能验证》**
*作者：Tanaka K. et al. (2018)*
摘要：利用体外纯化的FOXO1重组蛋白，证明其通过激活促凋亡基因Bim的表达诱导肝癌细胞凋亡，并揭示AKT磷酸化修饰对其活性的抑制作用。
3. **《基于重组FOXO1的高通量药物筛选平台开发》**
*作者：Chen L. et al. (2021)*
摘要：构建了基于荧光标记FOXO1重组蛋白的体外激酶活性检测系统，筛选出3种小分子化合物可增强FOXO1的核定位，潜在应用于糖尿病治疗。
(注：以上内容为示例模板，实际文献需通过PubMed/Google Scholar以"FOXO1 recombinant protein"为关键词检索，并筛选涉及蛋白表达、功能或应用的研究。)
Forkhead box protein O1 (FOXO1) is a member of the evolutionarily conserved FOXO transcription factor family, which plays pivotal roles in regulating cellular processes such as apoptosis, metabolism, oxidative stress response, and cell cycle progression. FOXO1 is primarily regulated by the insulin/PI3K/AKT signaling pathway; phosphorylation by AKT promotes its cytoplasmic retention and degradation, thereby inhibiting its transcriptional activity. Dysregulation of FOXO1 has been linked to metabolic disorders (e.g., diabetes), cancer, and aging-related diseases, making it a key therapeutic target.
Recombinant FOXO1 protein, engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to study its molecular mechanisms, post-translational modifications (e.g., phosphorylation, acetylation), and interactions with DNA or partner proteins. The recombinant form typically retains functional domains, including the forkhead DNA-binding domain and regulatory regions critical for nuclear localization and transcriptional activation. Researchers employ techniques like EMSA, ChIP, and luciferase assays to analyze its DNA-binding specificity and activity. Additionally, recombinant FOXO1 facilitates high-throughput drug screening for compounds modulating its activity. Despite challenges in maintaining stability and post-translational modification fidelity in vitro, advances in protein engineering (e.g., fusion tags, codon optimization) have improved its utility in structural studies (e.g., crystallography) and mechanistic research. This tool continues to enhance our understanding of FOXO1's role in health and disease.
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