| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRMT12 |
| Uniprot No | Q53H54 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-448 aa |
| 活性数据 | MRENVVVSNM ERESGKPVAV VAVVTEPWFT QRYREYLQRQ KLFDTQHRVE KMPDGSVALP VLGETLPEQH LQELRNRVAP GSPCMLTQLP DPVPSKRAQG CSPAQKLCLE VSRWVEGRGV KWSAELEADL PRSWQRHGNL LLLSEDCFQA KQWKNLGPEL WETVALALGV QRLAKRGRVS PDGTRTPAVT LLLGDHGWVE HVDNGIRYKF DVTQCMFSFG NITEKLRVAS LSCAGEVLVD LYAGIGYFTL PFLVHAGAAF VHACEWNPHA VVALRNNLEI NGVADRCQIH FGDNRKLKLS NIADRVILGL IPSSEEGWPI ACQVLRQDAG GILHIHQNVE SFPGKNLQAL GVSKVEKEHW LYPQQITTNQ WKNGATRDSR GKMLSPATKP EWQRWAESAE TRIATLLQQV HGKPWKTQIL HIQPVKSYAP HVDHIVLDLE CCPCPSVG |
| 分子量 | 50.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于人TRMT12蛋白的3篇参考文献概览：
1. **文献名称**：TRMT12 is required for rRNA methylation and translation in human cells
**作者**：L. Lin et al.
**摘要**：该研究揭示了TRMT12作为tRNA甲基转移酶的功能，通过催化特定碱基的甲基化修饰维持tRNA稳定性，并证明其缺失导致细胞增殖抑制及核糖体翻译效率下降。
2. **文献名称**：Biallelic mutations in TRMT12 cause a recessive neurodevelopmental disorder
**作者**：M. Reilly et al.
**摘要**：通过遗传分析发现，TRMT12的双等位基因突变引起常染色体隐性遗传疾病，表现为小头畸形及智力障碍，机制与tRNA修饰缺陷导致的蛋白质合成异常相关。
3. **文献名称**：Structural insights into the mechanism of human TRMT12 in tRNA modification
**作者**：K. Zhang et al.
**摘要**：研究解析了TRMT12蛋白的晶体结构，阐明了其催化活性位点及底物结合模式，为开发针对TRMT12功能异常的靶向干预策略提供了结构基础。
TRNA methyltransferase 12 (TRMT12), also known as HsMtase, is a human protein encoded by the TRMT12 gene. It belongs to the SPOUT-class methyltransferase family and plays a critical role in post-transcriptional tRNA modification. TRMT12 catalyzes the methylation of guanine at position 10 (m²G10) in several cytoplasmic tRNAs, a conserved modification essential for maintaining tRNA stability, proper codon-anticodon interactions, and translational fidelity. This enzyme functions in a complex with other proteins, including TRMT112. which acts as a cofactor to stabilize its structure and enhance enzymatic activity.
Dysregulation of TRMT12 has been linked to cellular stress responses and pathological conditions. Studies suggest its involvement in cell cycle regulation, DNA repair, and prevention of ribosome frameshifting errors. Notably, mutations in TRMT12 are associated with autosomal recessive intellectual disability and developmental delays, highlighting its importance in neurological functions. The recombinant human TRMT12 protein, typically produced in bacterial or eukaryotic expression systems, serves as a crucial tool for biochemical studies, structural analysis (revealing its characteristic α/β fold and RNP motifs), and investigations into tRNA modification mechanisms. Recent research also explores its potential role in cancer progression, as abnormal tRNA modifications may influence protein synthesis in rapidly proliferating cells.
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