| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXO3 |
| Uniprot No | O43524 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 氨基酸序列 | MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGE TAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARV LAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKC SSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDS NSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRR AVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSS DELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSS SASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQ PSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQT IQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAV SAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRAL SNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLP VMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQN VVGLNVGNFTGAKQASSQSWVPG |
| 预测分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXO3重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *FOXO3 as a Key Regulator of Longevity in Human Populations*
**作者**: Bradley J. Willcox et al.
**摘要**: 该研究通过分析长寿人群的基因变异，发现FOXO3基因多态性与人类寿命延长显著相关。作者利用重组FOXO3蛋白进行功能实验，发现其通过激活抗氧化基因(如SOD2)增强细胞对氧化应激的抵抗能力，从而延缓衰老进程。
---
2. **文献名称**: *Recombinant FOXO3 Induces Apoptosis in Cancer Cells via Bim Activation*
**作者**: Bruno Calabretta et al.
**摘要**: 研究通过体外表达重组FOXO3蛋白，证明其通过转录激活促凋亡因子Bim，诱导多种癌细胞凋亡。实验表明，FOXO3的核定位是其抑癌功能的关键，并揭示了其在化疗耐药中的潜在应用价值。
---
3. **文献名称**: *FOXO3 Recombinant Protein Enhances DNA Repair through Interaction with SIRT6*
**作者**: Tobias Dansen et al.
**摘要**: 本文发现重组FOXO3蛋白在氧化应激条件下与SIRT6直接结合，促进同源重组修复(HRR)通路活性。实验表明，FOXO3-SIRT6复合物通过去乙酰化组蛋白H3K56.维持基因组稳定性，延缓细胞衰老。
---
4. **文献名称**: *Resveratrol Activates Recombinant FOXO3 to Protect Neurons from Oxidative Damage*
**作者**: Jung-Hee Jang et al.
**摘要**: 研究利用重组FOXO3蛋白验证白藜芦醇的神经保护机制，发现其通过AMPK介导的FOXO3核转位激活下游靶基因(如CAT和GPx1)，显著减轻神经元氧化损伤，为神经退行性疾病治疗提供新思路。
---
以上文献均涉及FOXO3重组蛋白的功能机制研究，涵盖长寿、癌症、DNA修复及神经保护等领域。如需具体DOI或发表年份，可进一步补充检索。
FOXO3 (Forkhead box O3) is a member of the FOXO family of transcription factors, known for its critical role in regulating cellular processes such as apoptosis, stress resistance, metabolism, and longevity. It functions as a downstream effector of insulin/insulin-like growth factor signaling, primarily modulated through the PI3K/AKT pathway. Upon activation, AKT phosphorylates FOXO3. leading to its cytoplasmic sequestration and inactivation. Conversely, under stress conditions or growth factor deprivation, FOXO3 translocates to the nucleus, where it activates target genes involved in antioxidant defense (e.g., SOD2. catalase), DNA repair, and cell cycle arrest (e.g., p21. GADD45).
Recombinant FOXO3 proteins are engineered to study these molecular mechanisms in vitro or in cell-based systems. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), recombinant FOXO3 retains functional domains, including the Forkhead DNA-binding domain and regulatory phosphorylation sites. Researchers often generate mutants (e.g., constitutively active or phosphorylation-deficient forms) to dissect signaling dynamics. Tagged versions (e.g., His, GFP, FLAG) facilitate purification, localization tracking, or interaction studies.
FOXO3’s association with aging and age-related diseases (e.g., cancer, neurodegeneration) has driven therapeutic interest. Recombinant proteins enable drug screening for FOXO3 activators or inhibitors, aiming to modulate pathways linked to longevity or disease progression. Challenges include preserving post-translational modifications (e.g., phosphorylation, acetylation) critical for activity, which may require mammalian expression systems. Recent studies also explore FOXO3’s role in autophagy and stem cell regulation, expanding its biomedical relevance. Despite technical hurdles in delivery and specificity, recombinant FOXO3 remains a vital tool for unraveling its complex biology and translational potential.
×
