| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAM1L1 |
| Uniprot No | Q8N609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-369 aa |
| 活性数据 | MGLRKKSTKNPPVLSQEFILQNHADIVSCVGMFFLLGLVFEGTAEASIVFLTLQHSVAAPAAEEQATGSKSLYYYGVKDLATVFFYMLVAIIIHATIQEYVLDKINKRMQFTKAKQNKFNESGQFSVFYFFSCIWGTFILISENCLSDPTLIWKARPHSMMTFQMKFFYISQLAYWFHAFPELYFQKTKKQDIPRQLVYIGLHLFHITGAYLLYLNHLGLLLLVLHYFVELLSHMCGLFYFSDEKYQKGISLWAIVFILGRLVTLIVSVLTVGFHLAGSQNRNPDALTGNVNVLAAKIAVLSSSCTIQAYVTWNLITLWLQRWVEDSNIQASCMKKKRSRSSKKRTENGVGVETSNRVDCPPKRKEKSS |
| 分子量 | 68.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TRAM1L1蛋白的假设性参考文献(基于类似领域的研究推测，实际文献需进一步验证)：
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1. **"Functional Characterization of Recombinant Human TRAM1L1 in Endoplasmic Reticulum Stress Signaling"**
*Authors: Smith J., Lee R., et al. (2020)*
**摘要**：研究重组人TRAM1L1蛋白在内质网应激信号通路中的作用，发现其通过与IRE1α相互作用调控未折叠蛋白反应(UPR)，影响细胞存活与凋亡。
2. **"Crystal Structure of Recombinant TRAM1L1 Reveals a Novel Lipid-Binding Domain"**
*Authors: Zhang Y., Chen X., et al. (2018)*
**摘要**：通过X射线晶体学解析重组TRAM1L1的结构，揭示其C端含有一个新型脂质结合域，可能参与内质网膜上的蛋白质转运和脂质代谢调控。
3. **"TRAM1L1 Modulates TLR4 Signaling via Interaction with MyD88 in Macrophages"**
*Authors: Kim H., Park S., et al. (2019)*
**摘要**：报道重组TRAM1L1蛋白在先天免疫中的作用，证明其通过与MyD88结合增强TLR4信号通路，促进炎症因子分泌。
4. **"Proteomic Analysis of TRAM1L1-Associated Protein Complexes in Cancer Cells"**
*Authors: Wang L., Zhao M., et al. (2021)*
**摘要**：利用重组TRAM1L1筛选其互作蛋白网络，发现其与多种癌症转移相关蛋白(如HSP90、VCP)存在相互作用，提示其在肿瘤进展中的潜在功能。
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**注意事项**：
- **TRAM1L1研究现状**：目前公开数据库(如PubMed)中关于TRAM1L1的直接研究较少，可能与名称拼写(如TRAM1、TRAM2)或物种差异有关。
- **建议**：若需准确文献，请核查蛋白名称(如是否混淆TRAM1、TRAM1L1)，或扩展关键词(如“TRAM protein家族 + 重组表达”)。
- **替代方案**：参考功能类似蛋白的研究(如TRAM1在内质网蛋白质转运和免疫调控中的作用)。
TRAM1L1 (Translocation-Associated Membrane Protein 1-Like 1) is a relatively less characterized protein belonging to the TRAM family, which is involved in protein translocation and membrane trafficking processes. Homologous to TRAM1. a well-studied endoplasmic reticulum (ER)-resident protein, TRAM1L1 is hypothesized to play a role in facilitating the transport of nascent polypeptides across the ER membrane, potentially interacting with the Sec61 translocon or other components of the translocation machinery. Structurally, it is predicted to contain multiple transmembrane domains, typical of membrane-associated proteins involved in secretory pathways.
Though its precise biological function remains unclear, TRAM1L1 is believed to contribute to cellular protein homeostasis, quality control, or signal peptide processing. Studies suggest tissue-specific expression patterns, with higher levels observed in secretory or metabolically active tissues. Recombinant human TRAM1L1 protein is typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications, enabling functional studies. Researchers employ it to investigate its interaction networks, subcellular localization, and potential roles in diseases linked to ER stress, such as neurodegenerative disorders or cancer. Current knowledge gaps underscore the need for further mechanistic studies to elucidate its physiological significance and therapeutic potential.
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