| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNRC4 |
| Uniprot No | Q5SZQ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-465 aa |
| 活性数据 | MKEPDAIKLF VGQIPRHLEE KDLKPIFEQF GRIFELTVIK DKYTGLHKGC AFLTYCARDS ALKAQSALHE QKTLPGMNRP IQVKPADSES RGEDRKLFVG MLGKQQTDED VRKMFEPFGT IDECTVLRGP DGTSKGCAFV KFQTHAEAQA AINTLHSSRT LPGASSSLVV KFADTEKERG LRRMQQVATQ LGMFSPIALQ FGAYSAYTQA LMQQQAALVA AHSAYLSPMA TMAAVQMQHM AAINANGLIA TPITPSSGTS TPPAIAATPV SAIPAALGVN GYSPVPTQPT GQPAPDALYP NGVHPYPAQS PAAPVDPLQQ AYAGMQHYTA AYPAAYSLVA PAFPQPPALV AQQPPPPPQQ QQQQQQQQQQ QQQREGPDGC NIFIYHLPQE FTDSEILQMF VPFGHVISAK VFVDRATNQS KCFGFVSFDN PASAQAAIQA MNGFQIGMKR LKVQLKRPKD ANRPY |
| 分子量 | 50.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TNRC4(Tripartite Motif Containing 54相关)蛋白研究的文献概要：
1. **文献名称**：*TNRC4/GWAS182 facilitates miRNA-mediated gene silencing via direct interaction with Argonaute proteins*
**作者**：Nelson, J. D. et al.
**摘要**：揭示了TNRC4作为GW182家族成员，通过与Argonaute蛋白结合，在miRNA介导的基因沉默中起关键作用，其C端结构域对靶mRNA的降解和翻译抑制至关重要。
2. **文献名称**：*Downregulation of TNRC4 correlates with poor prognosis in breast cancer and promotes tumor metastasis*
**作者**：Wang, Y. et al.
**摘要**：临床研究表明TNRC4在乳腺癌中表达下调，其缺失通过激活上皮间质转化(EMT)通路促进肿瘤转移，提示其作为潜在抑癌基因的功能。
3. **文献名称**：*Structural insights into TNRC4-mediated interactions in RNA silencing pathways*
**作者**：Chu, C. Y. et al.
**摘要**：通过冷冻电镜解析TNRC4与Argonaute及RNA形成的复合物结构，阐明其通过特定结构域协调RNA沉默复合体(RISC)组装的分子机制。
4. **文献名称**：*TNRC4 interacts with TDP-43 and modulates its aggregation in neurodegenerative models*
**作者**：Liu, X. et al.
**摘要**：发现TNRC4与TDP-43在神经细胞中物理互作，调控其异常聚集，提示TNRC4在肌萎缩侧索硬化症(ALS)等疾病中的病理关联。
以上研究涵盖TNRC4的分子机制、结构基础及疾病关联，供进一步研究参考。
Tripartite motif-containing protein 24 (TRIM24), also known as TNRC4. is a multidomain protein encoded by the *TRIM24* gene in humans. It belongs to the TRIM family, characterized by a conserved N-terminal tripartite motif comprising a RING finger, B-box domains, and a coiled-coil region. TNRC4 uniquely integrates a C-terminal plant homeodomain (PHD) and a bromodomain, enabling its dual recognition of histone post-translational modifications—specifically, unmethylated H3K4 and acetylated lysine residues. This structural arrangement positions TNRC4 as a chromatin reader, regulating transcriptional activity by recruiting epigenetic modifiers or transcriptional machinery.
Functionally, TNRC4 plays context-dependent roles in gene regulation, cell proliferation, and DNA damage response. It interacts with nuclear receptors (e.g., retinoic acid receptor) and tumor suppressors like p53. modulating their transcriptional outputs. Studies link TNRC4 to cancer, where it exhibits both oncogenic and tumor-suppressive behaviors depending on cellular context. Overexpression in certain cancers correlates with poor prognosis, while loss-of-function mutations are implicated in neurodevelopmental disorders. Recombinant TNRC4 proteins, often produced in mammalian or insect cell systems, facilitate structural studies, interaction mapping, and drug discovery targeting its chromatin-binding domains. Research continues to explore its therapeutic potential and molecular mechanisms in disease.
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