| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MyoD |
| Uniprot No | P15172 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-320aa |
| 氨基酸序列 | 29aa_Tag_ELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRF FEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGR CLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQ RLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEH YSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGK SAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGE SSGDPTQSPDAAPQCPAGANPNPIYQVLLEESGGGGSPGRRRRRRRRRRR |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MyoD重组蛋白的3篇经典文献及其摘要概括：
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1. **文献名称**: *Expression of a single transfected cDNA converts fibroblasts to myoblasts*
**作者**: Davis, R.L.; Weintraub, H.; Lassar, A.B.
**摘要**: 该研究首次证实，通过转染MyoD的cDNA至成纤维细胞，可将其直接转化为具有收缩功能的肌细胞，揭示了MyoD作为肌肉生成主调控因子的关键作用，为重组MyoD蛋白在细胞重编程中的应用奠定基础。
2. **文献名称**: *Recombinant MyoD induces the activation of muscle-specific enhancers in non-muscle cells*
**作者**: Tapscott, S.J.; Lassar, A.B.; Weintraub, H.
**摘要**: 研究利用重组MyoD蛋白，证明其能够直接结合肌肉特异性基因的增强子区域(如肌酸激酶基因)，激活下游转录，揭示了MyoD通过E-box顺式元件调控肌肉分化的分子机制。
3. **文献名称**: *Crystal structure of MyoD bHLH domain-DNA complex: A basis for understanding myogenic conversions*
**作者**: Ma, P.C.; Rould, M.A.; Weintraub, H.; Pabo, C.O.
**摘要**: 通过X射线晶体学解析MyoD蛋白的bHLH结构域与DNA复合物的三维结构，阐明了其特异性识别E-box序列的结构基础，为设计重组MyoD突变体以优化功能提供理论支持。
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上述文献涵盖了MyoD的功能验证、分子机制及结构解析，均为该领域的里程碑研究。如需具体应用类文献(如肌肉再生或疾病治疗)，建议补充实验方向或年份范围以进一步筛选。
MyoD recombinant protein is a key tool in studying skeletal muscle development and cellular reprogramming. MyoD, a member of the myogenic regulatory factor (MRF) family, functions as a master transcription factor that initiates myogenesis by binding to specific DNA sequences (E-box motifs) to activate muscle-specific gene expression. Discovered in 1987. MyoD can convert non-muscle cells (e.g., fibroblasts) into myoblasts, highlighting its role in cell fate determination. Structurally, it contains a basic helix-loop-helix (bHLH) domain critical for DNA binding and dimerization, often partnering with E-proteins like E12/E47.
Recombinant MyoD is produced using expression systems (e.g., *E. coli* or mammalian cells) engineered to express purified MyoD protein with high stability and bioactivity. This allows precise control in experimental settings, bypassing variability from endogenous protein levels. Researchers use it to investigate myogenesis mechanisms, muscle regeneration pathways, and diseases like muscular dystrophy or sarcopenia. In regenerative medicine, MyoD recombinant protein is explored for directing stem cell differentiation into muscle lineages. Its ability to override cellular signaling pathways (e.g., Wnt or Notch) that suppress myogenesis makes it valuable for both basic research and therapeutic development. However, challenges remain in optimizing delivery methods and avoiding unintended oncogenic effects due to its potent transcriptional activity. Despite this, MyoD remains a cornerstone model for understanding transcription factor dynamics and cell lineage reprogramming.
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