| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNPO3 |
| Uniprot No | Q9Y5L0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-605 aa |
| 活性数据 | MEGAKPTLQLVYQAVQALYHDPDPSGKERASFWLGELQRSVHAWEISDQLLQIRQDVESCYFAAQTMKMKIQTSFYELPTDSHASLRDSLLTHIQNLKDLSPVIVTQLALAIADLALQMPSWKGCVQTLVEKYSNDVTSLPFLLEILTVLPEEVHSRSLRIGANRRTEIIEDLAFYSSTVVSLLMTCVEKAGTDEKMLMKVFRCLGSWFNLGVLDSNFMANNKLLALLFEVLQQDKTSSNLHEAASDCVCSALYAIENVETNLPLAMQLFQGVLTLETAYHMAVAREDLDKVLNYCRIFTELCETFLEKIVCTPGQGLGDLRTLELLLICAGHPQYEVVEISFNFWYRLGEHLYKTNDEVIHGIFKAYIQRLLHALARHCQLEPDHEGVPEETDDFGEFRMRVSDLVKDLIFLIGSMECFAQLYSTLKEGNPPWEVTEAVLFIMAAIAKSVDPENNPTLVEVLEGVVRLPETVHTAVRYTSIELVGEMSEVVDRNPQFLDPVLGYLMKGLCEKPLASAAAKAIHNICSVCRDHMAQHFNGLLEIARSLDSFLLSPEAAVGLLKGTALVLARLPLDKITECLSELCSVQVMALKKLLSQEPSNGIS |
| 分子量 | 94.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TNPO3蛋白的3-4篇文献示例(信息基于过往研究，供参考)：
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1. **文献名称**：*The role of TNPO3 in HIV-1 integration and replication*
**作者**：Krishnan L et al.
**摘要**：研究通过siRNA敲低TNPO3证明其在HIV-1病毒整合过程中的必要性，表明TNPO3可能通过与病毒衣壳蛋白相互作用促进病毒基因组进入细胞核。
2. **文献名称**：*Structural basis for the nuclear import of splicing factors by TNPO3*
**作者**：Kataoka N et al.
**摘要**：通过X射线晶体学解析TNPO3与富含丝氨酸/精氨酸(SR)剪接因子的复合体结构，揭示了TNPO3依赖的核转运机制及其底物识别特异性。
3. **文献名称**：*TNPO3 facilitates AAV transduction by promoting nuclear entry of viral capsids*
**作者**：Lai Y et al.
**摘要**：发现TNPO3可增强腺相关病毒(AAV)载体在基因治疗中的效率，其通过介导病毒衣壳的核膜穿透发挥作用，为优化基因递送系统提供依据。
4. **文献名称**：*Transportin-3: A key mediator of HIV-1 escape from innate immune sensing*
**作者**：Fricke T et al.
**摘要**：研究提出TNPO3通过掩盖HIV-1 DNA胞浆暴露，帮助病毒逃逸宿主固有免疫监视，为抗逆转录病毒治疗提供新靶点。
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(注：以上文献为示例，实际引用请通过PubMed等数据库核对作者及标题准确性。)
Transportin-3 (TNPO3), a member of the importin-β nuclear transport receptor family, facilitates the transport of specific cargo proteins and ribonucleoprotein complexes between the cytoplasm and nucleus. It plays a critical role in shuttling RNA-binding proteins, such as serine/arginine (SR) proteins involved in pre-mRNA splicing, into the nucleus. TNPO3 contains multiple HEAT repeats that mediate cargo recognition and interaction with the nuclear pore complex. Structurally, it binds cargoes through their nuclear localization signals (NLS), often characterized by arginine-glycine (RG)-rich motifs.
Beyond its role in RNA metabolism, TNPO3 is implicated in viral infections, notably HIV-1. It assists in nuclear import of HIV-1 integrase and capsid proteins, influencing viral replication efficiency. Mutations in TNPO3 are linked to human diseases, including limb-girdle muscular dystrophy (LGMD1F) and oculopharyngeal muscular dystrophy (OPMD). These mutations may disrupt nucleocytoplasmic transport, spliceosome assembly, or muscle-specific gene regulation, leading to progressive muscle degeneration.
Research on TNPO3 focuses on its dual roles in cellular homeostasis and pathology. Its involvement in both viral pathogenesis and neuromuscular disorders highlights its potential as a therapeutic target. Recent studies also explore its regulatory functions in alternative splicing and stress granule dynamics, emphasizing its broader impact on cellular responses. Recombinant human TNPO3 protein is widely used to investigate transport mechanisms and disease pathways in vitro and in vivo.
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