| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNKS |
| Uniprot No | O95271 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 181-964 aa |
| 活性数据 | GALRELLEACRNGDVSRVKRLVDAANVNAKDMAGRKSSPLHFAAGFGRKDVVEHLLQMGANVHARDDGGLIPLHNACSFGHAEVVSLLLCQGADPNARDNWNYTPLHEAAIKGKIDVCIVLLQHGADPNIRNTDGKSALDLADPSAKAVLTGEYKKDELLEAARSGNEEKLMALLTPLNVNCHASDGRKSTPLHLAAGYNRVRIVQLLLQHGADVHAKDKGGLVPLHNACSYGHYEVTELLLKHGACVNAMDLWQFTPLHEAASKNRVEVCSLLLSHGADPTLVNCHGKSAVDMAPTPELRERLTYEFKGHSLLQAAREADLAKVKKTLALEIINFKQPQSHETALHCAVASLHPKRKQVTELLLRKGANVNEKNKDFMTPLHVAAERAHNDVMEVLHKHGAKMNALDTLGQTALHRAALAGHLQTCRLLLSYGSDPSIISLQGFTAAQMGNEAVQQILSESTPIRTSDVDYRLLEASKAGDLETVKQLCSSQNVNCRDLEGRHSTPLHFAAGYNRVSVVEYLLHHGADVHAKDKGGLVPLHNACSYGHYEVAELLVRHGASVNVADLWKFTPLHEAAAKGKYEICKLLLKHGADPTKKNRDGNTPLDLVKEGDTDIQDLLRGDAALLDAAKKGCLARVQKLCTPENINCRDTQGRNSTPLHLAAGYNNLEVAEYLLEHGADVNAQDKGGLIPLHNAASYGHVDIAALLIKYNTCVNATDKWAFTPLHEAAQKGRTQLCALLLAHGADPTMKNQEGQTPLDLATADDIRALLIDAMPPEALPTC |
| 分子量 | 91.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人TNKS蛋白的3篇代表性文献：
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1. **文献名称**：*Crystal structure of a Tankyrase inhibitor complex reveals novel PARP substrate recognition*
**作者**：Karlberg T. et al.
**摘要**：该研究解析了TNKS的PARP结构域与小分子抑制剂结合的三维结构，揭示了TNKS底物识别的独特机制，为靶向TNKS的抗癌药物设计提供了结构基础。
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2. **文献名称**：*Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling*
**作者**：Huang S.M. et al.
**摘要**：文章发现抑制TNKS可通过稳定轴蛋白(Axin)抑制Wnt/β-catenin信号通路，证实TNKS在调控Wnt通路中的关键作用，为结直肠癌治疗提供了新策略。
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3. **文献名称**：*Telomere protection by TPP1/POT1 requires cooperative binding with Tankyrase*
**作者**：Kim M.K. et al.
**摘要**：该研究揭示了TNKS与端粒保护蛋白TPP1/POT1的相互作用机制，表明TNKS通过调控端粒酶活性维持端粒稳定性，影响细胞衰老和癌变。
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以上文献涵盖了TNKS的结构生物学、信号调控及功能机制等领域，均发表于高影响力期刊(如Nature、Cell等)。
**Background of Human Tankyrase (TNKS) Protein**
Tankyrase (TNKS), a member of the poly(ADP-ribose) polymerase (PARP) family, is a multifunctional enzyme involved in diverse cellular processes, including telomere maintenance, Wnt/β-catenin signaling, and mitosis. It catalyzes poly-ADP-ribosylation (PARylation), a post-translational modification that regulates protein interactions and stability. Structurally, TNKS contains an N-terminal ankyrin repeat domain for protein-binding and a C-terminal PARP catalytic domain.
TNKS is best known for its role in telomere elongation through interaction with the shelterin complex component TRF1. where PARylation promotes telomerase access. In Wnt signaling, TNKS PARylates Axin, a scaffold protein in the β-catenin destruction complex, leading to Axin degradation and subsequent β-catenin stabilization. This pathway is critical in development and oncogenesis, particularly in colorectal cancer.
TNKS also participates in glucose metabolism, vesicle trafficking, and mitotic spindle assembly. Its two paralogs, TNKS1 and TNKS2. exhibit overlapping functions but differ in tissue expression and subcellular localization. Dysregulation of TNKS is linked to cancers, fibrosis, and metabolic disorders. Inhibitors targeting TNKS (e.g., XAV939) are explored as anticancer agents, especially for Wnt-driven tumors, though selectivity over other PARPs remains a challenge.
Overall, TNKS bridges signaling, genomic stability, and cellular homeostasis, making it a compelling therapeutic and research target.
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