| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FRS2 |
| Uniprot No | Q8WU20 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-512aa |
| 氨基酸序列 | MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTR KRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNM LQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGY PRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTT GVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVK FVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPS VNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYE NLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTV PASAHKIEYSRRRDCTPTVFNFDIRRPSLEHRQLNYIQVDLEGGSDSDNP QTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSNLQKALPRDDGTSRKTR HNSTDLPMLAWK |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FRS2重组蛋白的参考文献(信息基于公开研究领域知识整合，非真实文献，仅供示例参考)：
1. **文献名称**: *"Structural insights into FRS2α-FGFR1 interaction through recombinant protein analysis"*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组表达FRS2α蛋白片段，结合X射线晶体学解析了其与FGFR1激酶结构域的相互作用界面，揭示了FRS2在受体酪氨酸激酶信号转导中的构象变化机制。
2. **文献名称**: *"Functional characterization of FRS2β recombinant protein in MAPK pathway activation"*
**作者**: Lee S, Park H.
**摘要**: 利用大肠杆菌系统表达纯化FRS2β重组蛋白，通过体外磷酸化实验证实其作为支架蛋白招募Grb2和SOS1.进而激活下游MAPK信号通路，促进细胞增殖。
3. **文献名称**: *"Recombinant FRS2-SH2 domain inhibits tumor growth in vivo by disrupting RTK signaling"*
**作者**: Zhang Y, et al.
**摘要**: 构建FRS2的SH2结构域重组蛋白，证明其可通过竞争性结合磷酸化酪氨酸位点阻断生长因子受体(如FGFR、TrkA)与内源性FRS2的相互作用，抑制移植瘤模型中的肿瘤生长。
(注：以上为模拟文献，实际研究需通过PubMed/Google Scholar等平台以“FRS2 recombinant protein”等关键词检索。)
FRS2 (Fibroblast Growth Factor Receptor Substrate 2) is a key adaptor protein involved in intracellular signaling pathways, particularly those mediated by fibroblast growth factor receptors (FGFRs). It was initially identified as a substrate for FGFR tyrosine kinase activity, playing a critical role in bridging receptor activation to downstream signaling cascades. Structurally, FRS2 contains an N-terminal myristoylation site for membrane localization, a phosphotyrosine-binding (PTB) domain that interacts with activated FGFRs, and multiple tyrosine phosphorylation sites that recruit signaling molecules like Grb2 and SOS1. thereby activating the MAPK/ERK pathway. Additionally, FRS2 facilitates cross-talk between FGFRs and other receptors, such as Ret and EGFR, amplifying its regulatory scope in cellular processes.
Recombinant FRS2 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms of the protein for research. These proteins retain critical domains necessary for studying FGFR signaling mechanisms, protein-protein interactions, and pathway modulation. They are widely used in *in vitro* assays, structural studies, and drug discovery to explore FRS2's role in development, tissue homeostasis, and disease. Dysregulation of FRS2 is implicated in cancers, developmental disorders, and metabolic syndromes, making it a target for therapeutic interventions. Recombinant FRS2 enables researchers to dissect its molecular functions and test inhibitors or activators in controlled settings, advancing understanding of FGFR-related pathologies and potential treatments.
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