| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FGF11 |
| Uniprot No | Q92914 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-225aa |
| 氨基酸序列 | MAALASSLIR QKREVREPGG SRPVSAQRRV CPRGTKSLCQ KQLLILLSKV RLCGGRPARP DRGPEPQLKG IVTKLFCRQG FYLQANPDGS IQGTPEDTSS FTHFNLIPVG LRVVTIQSAK LGHYMAMNAE GLLYSSPHFT AECRFKECVF ENYYVLYASA LYRQRRSGRA WYLGLDKEGQ VMKGNRVKKT KAAAHFLPKL LEVAMYQEPS LHSVPEASPS SPPAP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FGF11重组蛋白的模拟参考文献示例(注：以下内容为假设性文献，实际研究中请核实真实文献)：
1. **文献名称**: *"Functional characterization of recombinant human FGF11 in neuronal development"*
**作者**: Zhang, L. et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达并纯化了重组人FGF11蛋白，发现其通过激活MAPK信号通路促进神经元突触生长，提示其在神经发育中的潜在作用。
2. **文献名称**: *"Expression and purification of FGF11 in E. coli: Structural insights and stability analysis"*
**作者**: Kumar, S. & Lee, H.
**摘要**: 报道了一种高效的大肠杆菌表达系统，通过His标签亲和层析纯化FGF11.并利用圆二色谱分析其二级结构，证明重组蛋白在体外具有稳定性。
3. **文献名称**: *"FGF11 recombinant protein suppresses apoptosis in cardiomyocytes under hypoxic conditions"*
**作者**: Chen, Y. et al.
**摘要**: 研究显示，重组FGF11蛋白通过抑制线粒体途径凋亡蛋白(如Caspase-3)减轻心肌细胞缺氧损伤，为心血管疾病治疗提供新靶点。
4. **文献名称**: *"Development of a high-yield FGF11 recombinant protein production system using Pichia pastoris"*
**作者**: Rossi, M. et al.
**摘要**: 优化毕赤酵母表达体系，实现FGF11的高效分泌表达，并通过体外细胞增殖实验验证其生物活性，为规模化生产奠定基础。
(注：以上文献为示例性质，实际研究需参考PubMed、Web of Science等数据库中的真实文献。)
Fibroblast Growth Factor 11 (FGF11) is a member of the fibroblast growth factor (FGF) family, which comprises 23 structurally related proteins involved in diverse biological processes, including development, metabolism, and tissue repair. Unlike canonical FGFs (e.g., FGF1. FGF2) that act as secreted signaling molecules through cell-surface FGF receptors (FGFRs), FGF11 belongs to the FGF homologous factor (FHF) subfamily (FGF11-FGF14). These intracellular FGFs lack secretory signal peptides and are thought to function independently of FGFRs, primarily localizing to the cytoplasm and nucleus.
FGF11 is highly expressed in the nervous system, particularly in sensory neurons of the dorsal root ganglia and specific brain regions. Studies suggest its role in regulating neuronal excitability and ion channel activity. For example, FGF11 interacts with voltage-gated sodium channels (Nav1.7. Nav1.8) to modulate their trafficking and function, influencing pain signaling pathways. Dysregulation of FGF11 has been implicated in neuropathic pain, epilepsy, and neurodegenerative disorders, though its precise mechanisms remain under investigation.
Recombinant FGF11 protein is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to enable structural and functional studies. Its recombinant form allows researchers to explore intracellular interactions, post-translational modifications, and potential therapeutic applications. However, challenges persist in mimicking its native conformational state due to its intracellular nature and lack of traditional secretory pathways. Current research focuses on clarifying its physiological roles, signaling partners, and relevance to disease models, offering insights into novel targets for neurological and metabolic disorders.
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