| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZYX |
| Uniprot No | Q15942 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-572aa |
| 氨基酸序列 | MAAPRPSPAISVSVSAPAFYAPQEKFGPVVAPKPKVNPFRPGDSEPPPAP GAQRAQMGRVGEIPPPPPEDFPLPPPPLAGDGDDAEGALGGAFPPPPPPI EESFPPAPLEEEIFPSPPPPPEEEGGPEAPIPPPPQPREKVSSIDLEIDS LSSLLDDMTKNDPFKARVSSGYVPPPVATPFSSKSSTKPAAGGTAPLPPW KSPSSSQPLPQVPAPAQSQTQFHVQPQPQPKPQVQLHVQSQTQPVSLANT QPRGPPASSPAPAPKFSPVTPKFTPVASKFSPGAPGGSGSQPNQKLGHPE ALSAGTGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGP LTLKEVEELEQLTQQLMQDMEHPQRQNVAVNELCGRCHQPLARAQPAVRA LGQLFHIACFTCHQCAQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGEP ITDRMLRATGKAYHPHCFTCVVCARPLEGTSFIVDQANRPHCVPDYHKQY APRCSVCSEPIMPEPGRDETVRVVALDKNFHMKCYECEDCGKPLSIEADD NGCFPLDGHVLCRKCHTARAQT |
| 预测分子量 | 89 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZYX重组蛋白的模拟参考文献示例(注：内容为示例性概括，非真实文献)：
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1. **文献名称**：*"Recombinant ZYX Protein Expression and Its Role in Focal Adhesion Dynamics"*
**作者**：Chen L, et al.
**摘要**：本研究利用大肠杆菌系统成功表达并纯化了重组ZYX蛋白，分析了其与α-actinin和VASP的相互作用。实验表明，重组ZYX可调控细胞粘附斑的动态组装，为研究细胞迁移机制提供了工具。
2. **文献名称**：*"Structural Characterization of ZYX Domains Using Recombinant Protein Technology"*
**作者**：Smith J, Patel R.
**摘要**：通过X射线晶体学解析了重组ZYX蛋白LIM结构域的三维结构，揭示了其与整合素信号通路的结合位点，为靶向药物设计提供了结构基础。
3. **文献名称**：*"CRISPR/Cas9-Mediated ZYX Knockout Cells Combined with Recombinant ZYX Rescue Experiments"*
**作者**：Wang Y, et al.
**摘要**：在ZYX基因敲除的HEK293细胞中导入重组ZYX蛋白，证实其恢复细胞骨架张力及机械信号传导功能，证明ZYX在维持细胞力学稳态中的必要性。
4. **文献名称**：*"Recombinant ZYX as a Biomarker in Metastatic Cancer Cell Lines"*
**作者**：Kim S, et al.
**摘要**：通过体外实验发现，外源性重组ZYX蛋白能增强乳腺癌细胞的侵袭能力，提示其在癌症转移中的潜在作用及作为治疗靶点的可能性。
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(注：以上文献为模拟生成，实际引用需查询PubMed、Web of Science等数据库获取真实信息。)
Zyxin (ZYX) is a cytoskeletal protein primarily localized at focal adhesions and cell-cell junctions, where it plays a critical role in mechanotransduction, cell adhesion, and actin cytoskeleton remodeling. Initially identified as a component of adherens junctions in smooth muscle, ZYX is ubiquitously expressed across tissues and interacts with key structural proteins such as α-actinin, vinculin, and members of the Ena/VASP family. Structurally, it contains an N-terminal proline-rich domain for protein-protein interactions and three C-terminal LIM domains that mediate binding to integrins, transcription factors, and other signaling molecules. These features enable ZYX to act as a molecular scaffold, linking the extracellular matrix to intracellular signaling pathways and coordinating cellular responses to mechanical stress.
The dynamic shuttling of ZYX between the cytoplasm and nucleus under mechanical strain highlights its dual role in structural organization and gene regulation. Studies suggest it participates in stress fiber assembly, cell migration, and apoptosis regulation. Dysregulation of ZYX has been implicated in cancer metastasis, cardiovascular diseases, and fibrosis, where altered mechanosensing contributes to pathological tissue remodeling.
Recombinant ZYX protein production typically employs bacterial or mammalian expression systems to preserve post-translational modifications and functional domains. This engineered protein enables researchers to study ZYX's interactions, structure-function relationships, and mechanobiological roles in vitro. Its applications span from elucidating focal adhesion dynamics to screening potential therapeutics targeting mechanosensitive pathways. Recent advances in cryo-EM and structural biology have further driven interest in recombinant ZYX as a tool for decoding mechanochemical signaling networks at subcellular resolutions. Ongoing research explores its potential as a biomarker or therapeutic target in diseases characterized by aberrant mechanical signaling.
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