| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD300E |
| Uniprot No | Q496F6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-173aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSLKGPGSVTGTAGDSLTVWCQYESMYKG YNKYWCRGQYDTSCESIVETKGEEKVERNGRVSIRDHPEALAFTVTMQNL NEDDAGSYWCKIQTVWVLDSWSRDPSDLVRVYVSPAITTPRRTTHPATPP IFLVVNPGRNLSTGEVLTQNSGFRLSSPH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD300E重组蛋白的3篇参考文献的简要列举(注：文献为虚构示例，实际研究中需根据真实文献调整)：
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1. **文献名称**: "Recombinant CD300E Protein Enhances Macrophage Activation via TLR4 Synergy"
**作者**: Smith A, et al. (2020)
**摘要**: 该研究在HEK293细胞中表达了功能性CD300E重组蛋白，证实其通过与TLR4信号协同作用，促进巨噬细胞的炎症因子(如TNF-α、IL-6)释放，提示CD300E在先天免疫调控中的潜在机制。
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2. **文献名称**: "CD300E Binds Phosphatidylserine and Modulates Inflammatory Responses in Dendritic Cells"
**作者**: Zhang Y, et al. (2018)
**摘要**: 利用重组CD300E蛋白结合实验，作者发现其特异性识别磷脂酰丝氨酸(PS)，并通过激活Syk激酶通路增强树突状细胞的抗原呈递功能，为CD300E在感染或凋亡细胞清除中的作用提供了证据。
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3. **文献名称**: "Structural and Functional Characterization of Recombinant CD300E in Viral Infection Models"
**作者**: García-Sastre A, et al. (2021)
**摘要**: 通过晶体学解析CD300E重组蛋白的胞外结构域，并结合体外病毒感染实验，发现其通过调控I型干扰素(IFN-β)通路抑制病毒复制，提示CD300E可能作为抗病毒治疗的靶点。
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**备注**：以上文献为示例性内容，实际研究需查询PubMed、Web of Science等平台获取真实文献。若需进一步协助检索，请提供更多背景信息。
CD300E, a member of the CD300 cell surface receptor family, is a transmembrane glycoprotein predominantly expressed on myeloid cells, such as monocytes, macrophages, and dendritic cells, as well as platelets. This immunomodulatory protein belongs to the immunoglobulin (Ig) superfamily, characterized by a single extracellular Ig-like domain involved in ligand recognition and intercellular interactions. CD300E plays a critical role in regulating immune responses, particularly in balancing activating and inhibitory signals. It interacts with ligands like phosphatidylserine and phosphatidylethanolamine, which are exposed on apoptotic cells or pathogen membranes, suggesting its involvement in pathogen recognition, clearance of cellular debris, and inflammation modulation. Dysregulation of CD300E has been linked to inflammatory diseases, infections, and cancer progression.
Recombinant CD300E protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce soluble forms of the extracellular domain, often fused with tags (e.g., Fc, His-tag) for purification and functional studies. This protein serves as a vital tool to investigate receptor-ligand interactions, signaling pathways, and its role in immune regulation. Researchers utilize it in ELISA, flow cytometry, and cell-based assays to study binding kinetics, receptor dimerization, or competitive inhibition of native CD300E. Additionally, it aids in therapeutic antibody development and screening for agonists/antagonists targeting CD300E-related pathologies. Recent studies highlight its potential as a biomarker or therapeutic target in sepsis, autoimmune disorders, and tumor microenvironments, emphasizing its growing relevance in immunology and translational medicine.
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