| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD300C |
| Uniprot No | Q08708 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-183aa |
| 氨基酸序列 | GYFPLSHPMTVAGPVGGSLSVQCRYEKEHRTLNKFWCRPPQILRCDKIVE TKGSAGKRNGRVSIRDSPANLSFTVTLENLTEEDAGTYWCGVDTPWLRDF HDPIVEVEVSVFPAGTTTASSPQSSMGTSGPPTKLPVHTWPSVTRKDSPE PSPHPGSLFSNVRVDHHHHHH |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD300C重组蛋白的模拟参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：Structural and Functional Characterization of Recombinant CD300C in Immune Regulation
**作者**：Zhang L, et al.
**摘要**：本研究成功表达并纯化了人源CD300C重组蛋白，通过X射线晶体学解析其胞外结构域的三维结构，揭示了其与磷脂配体结合的关键位点，并证明其在巨噬细胞中调控炎症反应的作用。
2. **文献名称**：CD300C Recombinant Protein Modulates Allergic Responses via IgE Receptor Interaction
**作者**：Tanaka K, et al.
**摘要**：该研究利用重组CD300C蛋白探究其在过敏性疾病中的作用，发现其通过与肥大细胞表面IgE受体交联，抑制过度脱颗粒反应，为治疗过敏提供潜在靶点。
3. **文献名称**：Recombinant CD300C Enhances Bacterial Clearance in Sepsis Models
**作者**：Smith J, et al.
**摘要**：通过在脓毒症小鼠模型中注射重组CD300C蛋白，研究证明其通过促进中性粒细胞吞噬功能和减少细胞因子风暴，显著提高宿主对细菌感染的防御能力。
4. **文献名称**：Expression and Immunomodulatory Activity of CD300C in Viral Infections
**作者**：Wang Y, et al.
**摘要**：本研究构建了CD300C重组蛋白的真核表达系统，发现其在抗病毒天然免疫中通过调控干扰素信号通路，抑制流感病毒复制，并揭示其与TLR通路的协同作用。
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**注意**：以上文献及摘要均为模拟生成，真实文献需通过PubMed、Web of Science等数据库检索关键词“CD300C recombinant protein”或“CD300C immune function”获取。
CD300c, a member of the CD300 family of immunoregulatory cell-surface receptors, plays a role in modulating immune responses. This protein is part of a conserved group of receptors expressed primarily on myeloid cells, such as dendritic cells, monocytes, and macrophages. Structurally, CD300c contains an immunoglobulin (Ig)-like domain in its extracellular region, a transmembrane domain, and a short cytoplasmic tail lacking intrinsic signaling motifs. It interacts with ligands such as phosphatidylserine and phosphatidylethanolamine, which are exposed on apoptotic cells or pathogens, suggesting its involvement in phagocytosis, inflammation, or pathogen sensing.
Recombinant CD300c protein is engineered for experimental studies to elucidate its biological functions. Produced via heterologous expression systems (e.g., mammalian or insect cells), the recombinant form typically includes the extracellular domain fused with tags (e.g., Fc or His-tag) to facilitate purification and detection. This tool enables researchers to study ligand-receptor interactions, immune cell activation, and downstream signaling pathways. Its applications span in vitro binding assays, structural studies (e.g., crystallography), and therapeutic antibody development. Dysregulation of CD300 family members has been linked to autoimmune diseases, allergies, and cancer, making CD300c a potential target for immunomodulatory therapies. Current research focuses on clarifying its ligand specificity, signaling partners, and role in balancing activating/inhibitory immune signals. However, its exact mechanisms and physiological relevance remain less characterized compared to other CD300 proteins, underscoring the need for further investigation using recombinant tools.
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