| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD207 |
| Uniprot No | Q9UJ71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 65-328aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSPRFMGTI SDVKTNVQLL KGRVDNISTL DSEIKKNSDG MEAAGVQIQM VNESLGYVRS QFLKLKTSVE KANAQIQILT RSWEEVSTLN AQIPELKSDL EKASALNTKI RALQGSLENM SKLLKRQNDI LQVVSQGWKY FKGNFYYFSL IPKTWYSAEQ FCVSRNSHLT SVTSESEQEF LYKTAGGLIY WIGLTKAGME GDWSWVDDTP FNKVQSARFW IPGEPNNAGN NEHCGNIKAP SLQAWNDAPC DKTFLFICKR PYVPSEP |
| 预测分子量 | 32 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD207(Langerin)重组蛋白的3篇模拟参考文献及其摘要概括：
---
1. **文献名称**：*Expression and Functional Characterization of Recombinant Human Langerin (CD207) in Escherichia coli*
**作者**：Müller, C.E., et al.
**摘要**：该研究报道了在大肠杆菌中成功表达并纯化可溶性CD207重组蛋白，通过糖结合实验证实其保留了对甘露糖和β-葡聚糖的特异性识别能力，为研究其与病原体互作提供了工具。
---
2. **文献名称**：*Structural Insights into Langerin Glycan Binding via Crystallography of Recombinant CD207*
**作者**：Sato, T., & Fujimoto, K.
**摘要**：利用杆状病毒-昆虫细胞系统表达CD207重组蛋白，解析其晶体结构，揭示了碳水化合物识别域(CRD)的关键氨基酸残基如何介导病原体表面糖结构的结合。
---
3. **文献名称**：*Recombinant Langerin Fusion Protein Enhances Antigen Uptake in Dendritic Cell Vaccines*
**作者**：Zhang, L., et al.
**摘要**：构建了CD207-Fc重组融合蛋白，证明其可通过靶向递送抗原至树突状细胞，显著增强疫苗诱导的免疫反应，为基于CD207的免疫疗法提供了实验依据。
---
(注：以上文献为模拟内容，实际引用请检索PubMed等数据库获取真实文献。)
**Background of CD207 Recombinant Protein**
CD207. also known as langerin, is a C-type lectin receptor encoded by the *CD207* gene. It is primarily expressed on the surface of Langerhans cells (LCs), a specialized subset of dendritic cells located in the epidermis and mucosal epithelia. Functioning as a pattern recognition receptor, CD207 plays a critical role in immune surveillance by binding to pathogen-associated carbohydrate structures, such as mannose, fucose, and glucan. This interaction facilitates the recognition and internalization of pathogens, including HIV, *Candida albicans*, and herpes simplex virus (HSV), enabling antigen processing and presentation to T cells for adaptive immune activation.
Structurally, CD207 consists of a cytoplasmic tail, a transmembrane domain, and an extracellular carbohydrate recognition domain (CRD). Its unique ability to form Birbeck granules—cytoplasmic organelles involved in antigen capture and transport—further underscores its importance in immune responses. Recombinant CD207 protein is engineered through molecular cloning, typically expressed in mammalian or insect cell systems to ensure proper glycosylation and folding. Purification often involves affinity tags (e.g., His-tag) for high yield and purity.
Research applications of recombinant CD207 span infectious disease studies, vaccine development, and cancer immunotherapy. It serves as a tool to investigate host-pathogen interactions, screen inhibitory compounds, or design targeted vaccines. Additionally, its role in modulating immune tolerance and inflammation has implications for autoimmune and allergic disease research. By leveraging recombinant CD207. scientists aim to unravel its structural-functional dynamics and harness its potential in therapeutic strategies.
×
