| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RELA |
| Uniprot No | Q04206 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGD |
| 预测分子量 | 1-210aa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RELA重组蛋白的3篇参考文献示例(注：内容为模拟概括，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Prokaryotic Expression and Functional Characterization of Recombinant RELA/p50 Heterodimer for NF-κB Binding Studies"
**作者**: Chen L, et al.
**摘要**: 该研究报道了在大肠杆菌中高效表达并纯化RELA/p50异源二聚体重组蛋白，验证其与κB位点DNA结合的能力，为体外研究NF-κB调控机制提供工具。
2. **文献名称**: "Phosphomimetic Mutation of RELA at Ser536 Enhances Transcriptional Activation in Inflammatory Pathways"
**作者**: Smith JR, et al.
**摘要**: 通过构建RELA S536E磷酸化模拟突变体重组蛋白，证实该修饰显著提升RELA的转录活性，揭示其在炎症信号放大中的作用机制。
3. **文献名称**: "High-Throughput Screening of RELA Inhibitors Using Recombinant Protein-Based Luciferase Assay"
**作者**: Kim H, et al.
**摘要**: 开发基于重组RELA蛋白的荧光素酶报告系统，用于筛选靶向NF-κB通路的小分子抑制剂，鉴定出两种先导化合物可抑制RELA核转位。
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建议通过PubMed或Google Scholar搜索关键词“RELA recombinant protein”或“recombinant NFKB3”获取最新文献。
RELA, also known as p65. is a critical subunit of the nuclear factor kappa B (NF-κB) family of transcription factors, which play pivotal roles in regulating immune responses, inflammation, cell survival, and proliferation. Discovered in the 1980s, NF-κB is a dimeric protein complex, with RELA forming a heterodimer most commonly with p50 (NF-κB1). Unlike other NF-κB subunits, RELA contains a potent transactivation domain (TAD) at its C-terminus, enabling direct interaction with transcriptional machinery to activate target genes. In resting cells, RELA is sequestered in the cytoplasm by inhibitory proteins, such as IκB. Upon stimulation by cytokines, pathogens, or stress signals, IκB is degraded, freeing RELA to translocate into the nucleus and regulate gene expression.
Recombinant RELA protein is engineered for research and therapeutic applications. Produced via bacterial or mammalian expression systems, it retains functional domains: the N-terminal Rel homology domain (RHD) for DNA binding and dimerization, and the C-terminal TAD for transcriptional activation. Researchers utilize recombinant RELA to study NF-κB signaling mechanisms, screen inhibitors for inflammatory diseases or cancer, and develop gene therapy vectors. Its purity and activity are validated through techniques like EMSA, luciferase reporter assays, and Western blotting.
As dysregulated NF-κB signaling is implicated in chronic inflammation, autoimmune disorders, and tumorigenesis, recombinant RELA serves as a vital tool for dissecting pathological pathways and identifying targeted therapies. Its applications extend to structural studies, antibody development, and high-throughput drug screening, underscoring its importance in both basic and translational biomedical research.
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