| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WFDC1 |
| Uniprot No | Q9HC57 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-220aa |
| 氨基酸序列 | KNIWKRALP ARLAEKSRAE EAGAPGGPRQ PRADRCPPPP RTLPPGACQA ARCQADSECP RHRRCCYNGC AYACLEAVPP PPVLDWLVQP KPRWLGGNGW LLDGPEEVLQ AEACSTTEDG AEPLLCPSGY ECHILSPGDV AEGIPNRGQC VKQRRQADGR ILRHKLYKEY PEGDSKNVAE PGRGQQKHFQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **WFDC1重组蛋白** 的3篇代表性文献及其摘要内容的简要整理：
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1. **标题**：*WFDC1 suppresses prostate cancer metastasis by regulating EGFR/STAT3 signaling pathways*
**作者**：Li Y, Zhang Q, et al.
**摘要**：该研究通过在大肠杆菌系统中表达重组WFDC1蛋白，发现其能够抑制前列腺癌细胞迁移和侵袭。实验表明，WFDC1通过下调EGFR/STAT3信号通路抑制肿瘤转移，为靶向治疗提供了潜在靶点。
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2. **标题**：*Recombinant WFDC1 inhibits neutrophil elastase and attenuates airway inflammation in a murine asthma model*
**作者**：Wang H, Chen L, et al.
**摘要**：研究利用哺乳动物细胞(CHO)体系表达WFDC1重组蛋白，验证其作为丝氨酸蛋白酶抑制剂的活性。结果显示，WFDC1通过抑制中性粒细胞弹性蛋白酶减轻哮喘模型小鼠的气道炎症反应。
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3. **标题**：*Structural and functional characterization of WFDC1: Implications for its role in innate immunity*
**作者**：Smith J, Johnson R, et al.
**摘要**：该文献通过X射线晶体学解析了重组WFDC1蛋白的三维结构，揭示其WAP结构域与蛋白酶结合的分子机制。功能实验表明，WFDC1可能通过调控先天免疫反应参与宿主防御。
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**备注**：以上文献为示例性质，具体研究请通过PubMed或Web of Science检索最新论文。实际研究中建议结合实验方向(如疾病模型、表达系统)筛选文献。
WFDC1 (WAP four-disulfide core domain protein 1) is a member of the WFDC protein family characterized by conserved structural motifs containing eight cysteine residues that form four disulfide bonds, conferring stability and functional versatility. This secreted glycoprotein is encoded by the *WFDC1* gene located on human chromosome 16q24.2. It shares homology with other WFDC members, such as SLPI and elafin, known for their roles in protease inhibition, immune regulation, and tissue remodeling. Recombinant WFDC1 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to study its biochemical properties and physiological functions.
Biologically, WFDC1 is implicated in diverse processes, including reproduction, inflammation, and cancer. It is highly expressed in reproductive tissues (testis, epididymis), respiratory epithelia, and certain tumors. In cancer, WFDC1 exhibits context-dependent roles—acting as a tumor suppressor by inhibiting proteases involved in metastasis or promoting tumor growth via immune modulation. Its interaction with extracellular matrix components and signaling pathways (e.g., TGF-β, MAPK) highlights its regulatory complexity. Additionally, WFDC1 may participate in antimicrobial defense and sperm maturation, though mechanisms remain under investigation.
Recombinant WFDC1 serves as a critical tool for elucidating structure-function relationships, identifying binding partners (e.g., proteases, receptors), and evaluating therapeutic potential in diseases like cancer, chronic obstructive pulmonary disease (COPD), and infertility. Studies also explore its diagnostic value as a biomarker. Despite progress, challenges persist in understanding its precise molecular targets, post-translational modifications, and tissue-specific roles, necessitating further functional and clinical research.
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