| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WFS1 |
| Uniprot No | O76024 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-890aa |
| 氨基酸序列 | MDSNTAPLGPSCPQPPPAPQPQARSRLNATASLEQERSERPRAPGPQAGPGPGVRDAAAPAEPQAQHTRSRERADGTGPTKGDMEIPFEEVLERAKAGDPKAQTEVGKHYLQLAGDTDEELNSCTAVDWLVLAAKQGRREAVKLLRRCLADRRGITSENEREVRQLSSETDLERAVRKAALVMYWKLNPKKKKQVAVAELLENVGQVNEHDGGAQPGPVPKSLQKQRRMLERLVSSESKNYIALDDFVEITKKYAKGVIPSSLFLQDDEDDDELAGKSPEDLPLRLKVVKYPLHAIMEIKEYLIDMASRAGMHWLSTIIPTHHINALIFFFIVSNLTIDFFAFFIPLVIFYLSFISMVICTLKVFQDSKAWENFRTLTDLLLRFEPNLDVEQAEVNFGWNHLEPYAHFLLSVFFVIFSFPIASKDCIPCSELAVITGFFTVTSYLSLSTHAEPYTRRALATEVTAGLLSLLPSMPLNWPYLKVLGQTFITVPVGHLVVLNVSVPCLLYVYLLYLFFRMAQLRNFKGTYCYLVPYLVCFMWCELSVVILLESTGLGLLRASIGYFLFLFALPILVAGLALVGVLQFARWFTSLELTKIAVTVAVCSVPLLLRWWTKASFSVVGMVKSLTRSSMVKLILVWLTAIVLFCWFYVYRSEGMKVYNSTLTWQQYGALCGPRAWKETNMARTQILCSHLEGHRVTWTGRFKYVRVTDIDNSAESAINMLPFFIGDWMRCLYGEAYPACSPGNTSTAEEELCRLKLLAKHPCHIKKFDRYKFEITVGMPFSSGADGSRSREEDDVTKDIVLRASSEFKSVLLSLRQGSLIEFSTILEGRLGSKWPVFELKAISCLNCMAQLSPTRRHVKIEHDWRSTVHGAVKFAFDFFFFPFLSAA |
| 预测分子量 | 106.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WFS1重组蛋白研究的示例性参考文献(内容为模拟生成，建议通过学术数据库获取真实文献)：
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1. **文献名称**: *Expression and Functional Analysis of Recombinant WFS1 Protein in Cellular Models*
**作者**: Smith A, et al.
**摘要**: 研究利用大肠杆菌表达系统成功纯化WFS1重组蛋白，并验证其在调节内质网应激中的作用，发现WFS1缺失导致细胞凋亡增加，提示其在Wolfram综合征中的病理机制。
2. **文献名称**: *Structural Characterization of Human WFS1 Protein Using Recombinant Expression*
**作者**: Tanaka K, et al.
**摘要**: 通过哺乳动物细胞系表达WFS1重组蛋白，结合X射线晶体学解析其结构，揭示了跨膜结构域的关键功能区域，为突变导致的Wolfram综合征提供分子基础。
3. **文献名称**: *WFS1 Recombinant Protein Rescues Mitochondrial Dysfunction in Patient-Derived Cells*
**作者**: Chen L, et al.
**摘要**: 实验表明外源性添加WFS1重组蛋白可改善Wolfram综合征患者细胞中线粒体膜电位异常，证明其潜在治疗价值。
4. **文献名称**: *Development of a WFS1 Recombinant Protein-Based Assay for Drug Screening*
**作者**: Gupta R, et al.
**摘要**: 构建基于WFS1重组蛋白的高通量筛选平台，用于发现稳定内质网-线粒体交互作用的小分子化合物，推动Wolfram综合征药物开发。
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建议通过PubMed、Web of Science等平台以“WFS1 recombinant protein”为关键词检索最新研究。
**Background of WFS1 Recombinant Protein**
The WFS1 gene encodes wolframin, a transmembrane protein predominantly localized in the endoplasmic reticulum (ER). It plays a critical role in ER homeostasis, calcium regulation, and cellular stress responses. Mutations in WFS1 are linked to Wolfram syndrome, a rare autosomal recessive disorder characterized by diabetes mellitus, optic atrophy, and neurodegenerative features. The protein’s structural domains, including nine transmembrane regions, suggest its involvement in ER-associated processes such as protein folding and secretion.
Recombinant WFS1 protein is produced *in vitro* using expression systems (e.g., mammalian cells, bacteria) to study its function and interactions. Researchers utilize this tool to investigate disease mechanisms, particularly how loss-of-function mutations disrupt ER stress pathways, leading to β-cell apoptosis (in diabetes) or neuronal degeneration. Structural studies of recombinant WFS1 have identified key regions critical for its stability and interaction with partners like calreticulin and ER stress sensors.
Beyond basic research, recombinant WFS1 holds therapeutic potential. It aids in high-throughput drug screening to identify compounds that stabilize mutant wolframin or enhance ER stress resilience. Gene therapy approaches also leverage WFS1 cDNA to restore protein function in preclinical models. Challenges remain in optimizing recombinant protein folding and post-translational modifications to mimic native structures, which are vital for functional assays.
Overall, WFS1 recombinant protein serves as a cornerstone for unraveling Wolfram syndrome pathology and developing targeted interventions for ER-related disorders.
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