| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YAP1 |
| Uniprot No | P46937 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-504aa |
| 氨基酸序列 | MDPGQQPPPQPAPQGQGQPPSQPPQGQGPPSGPGQPAPAATQAAPQAPPA GHQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPE PKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSG PAATPTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQ DPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYIN HKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNS NQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRS QLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDS GLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPG TNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESF LTWL |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional analysis of the YAP1 protein"** by Sudol et al.
*摘要*：本研究通过重组表达纯化YAP1蛋白，结合X射线晶体学解析其结构，揭示了YAP1与TEAD转录因子结合的关键结构域，阐明了其在Hippo信号通路中的调控机制。
2. **"YAP1重组蛋白在细胞增殖与凋亡中的双重作用"** by Hansen et al.
*摘要*：通过体外重组YAP1蛋白实验，发现其磷酸化状态可调控与LATS1激酶的相互作用，进而影响下游靶基因表达，揭示YAP1在细胞命运决定中的动态平衡。
3. **"Mechanistic insights into YAP1-mediated oncogenesis"** by Pan et al.
*摘要*：利用重组YAP1蛋白进行功能分析，证明其过度激活可通过结合TEAD促进肿瘤细胞增殖和迁移，为靶向YAP1-TEAD相互作用的抗癌药物开发提供依据。
4. **"Recombinant YAP1 protein enhances tissue regeneration in vitro"** by Yu et al.
*摘要*：研究重组YAP1蛋白在干细胞中的外源性递送效果，发现其可激活促增殖基因并加速损伤修复，提示其在再生医学中的潜在应用价值。
(注：以上文献名为示例，作者及内容为虚构，实际需根据具体论文调整。)
**Background of YAP1 Recombinant Protein**
Yes-associated protein 1 (YAP1) is a critical transcriptional coactivator in the Hippo signaling pathway, a conserved regulatory network that controls organ size, tissue homeostasis, and cell proliferation. YAP1 functions as a downstream effector of this pathway, mediating cellular responses to mechanical cues, cell polarity, and metabolic signals. When the Hippo pathway is inactive, YAP1 translocates to the nucleus, where it interacts with transcription factors like TEAD (transcriptional enhancer associate domain) to drive the expression of genes promoting cell growth, survival, and stem cell maintenance. Conversely, Hippo pathway activation phosphorylates YAP1. leading to its cytoplasmic retention and degradation, thereby inhibiting proliferative signals.
Recombinant YAP1 protein is engineered through molecular cloning and expression systems (e.g., *E. coli*, mammalian, or insect cells*) to produce purified, functional YAP1 for research and therapeutic applications. This recombinant form retains key structural domains, including the WW domains for protein-protein interactions and the TEAD-binding region, enabling studies on its mechanistic roles in development, regeneration, and disease. Researchers utilize YAP1 recombinant protein to investigate its interplay with signaling pathways (e.g., Wnt, TGF-β) and its dual role in cancer—acting as an oncogene in some contexts (e.g., promoting tumor growth) or a tumor suppressor in others.
In regenerative medicine, YAP1’s ability to enhance tissue repair and stem cell expansion has spurred interest in leveraging recombinant YAP1 for therapeutic strategies. However, its oncogenic potential necessitates precise regulation. Studies also explore YAP1’s involvement in mechanotransduction, fibrosis, and immune modulation, highlighting its broad biological relevance. Recombinant YAP1 thus serves as a vital tool for dissecting Hippo pathway dynamics and developing targeted therapies for cancer, degenerative diseases, and tissue engineering.
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