| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EZH1 |
| Uniprot No | Q92800 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747aa |
| 氨基酸序列 | MEIPNPPTSK CITYWKRKVK SEYMRLRQLK RLQANMGAKA LYVANFAKVQ EKTQILNEEW KKLRVQPVQS MKPVSGHPFL KKCTIESIFP GFASQHMLMR SLNTVALVPI MYSWSPLQQN FMVEDETVLC NIPYMGDEVK EEDETFIEEL INNYDGKVHG EEEMIPGSVL ISDAVFLELV DALNQYSDEE EEGHNDTSDG KQDDSKEDLP VTRKRKRHAI EGNKKSSKKQ FPNDMIFSAI ASMFPENGVP DDMKERYREL TEMSDPNALP PQCTPNIDGP NAKSVQREQS LHSFHTLFCR RCFKYDCFLH PFHATPNVYK RKNKEIKIEP EPCGTDCFLL LEGAKEYAML HNPRSKCSGR RRRRHHIVSA SCSNASASAV AETKEGDSDR DTGNDWASSS SEANSRCQTP TKQKASPAPP QLCVVEAPSE PVEWTGAEES LFRVFHGTYF NNFCSIARLL GTKTCKQVFQ FAVKESLILK LPTDELMNPS QKKKRKHRLW AAHCRKIQLK KDNSSTQVYN YQPCDHPDRP CDSTCPCIMT QNFCEKFCQC NPDCQNRFPG CRCKTQCNTK QCPCYLAVRE CDPDLCLTCG ASEHWDCKVV SCKNCSIQRG LKKHLLLAPS DVAGWGTFIK ESVQKNEFIS EYCGELISQD EADRRGKVYD KYMSSFLFNL NNDFVVDATR KGNKIRFANH SVNPNCYAKV VMVNGDHRIG IFAKRAIQAG EELFFDYRYS QADALKYVGI ERETDVL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EZH1重组蛋白的3篇参考文献示例(内容为模拟，非真实文献)：
1. **文献名称**：*Structural and Functional Analysis of Recombinant EZH1 Reveals Distinct Roles in Histone Methylation*
**作者**：Smith A, et al.
**摘要**：研究解析了重组EZH1蛋白的晶体结构，发现其与EZH2相比具有差异化的底物结合域，体外实验表明EZH1在特定细胞类型中调控H3K27me3的效率较低，但可与PRC2亚基形成非经典复合物。
2. **文献名称**：*EZH1 Recombinant Protein Exhibits Context-Dependent Enzymatic Activity in Hematopoietic Stem Cells*
**作者**：Tanaka K, et al.
**摘要**：通过纯化重组EZH1蛋白，发现其在造血干细胞中与EZH2协同维持基因沉默，但在缺少EZH2时，EZH1单独表现出弱甲基转移酶活性，提示其在特定生理环境中的补偿功能。
3. **文献名称**：*Development of a High-Throughput Assay for Screening EZH1 Inhibitors Using Recombinant Protein*
**作者**：Chen L, et al.
**摘要**：研究利用重组EZH1蛋白建立体外高通量筛选平台，鉴定出小分子抑制剂可选择性靶向EZH1的SET结构域，为治疗EZH1异常激活的血液疾病提供潜在策略。
注：以上文献及内容为模拟生成，实际引用时需检索PubMed等数据库获取真实文献。
EZH1 (Enhancer of Zeste Homolog 1) is a catalytic subunit of the Polycomb Repressive Complex 2 (PRC2), a critical epigenetic regulator involved in gene silencing through histone modification. As a homolog of EZH2. EZH1 shares structural similarities, including a conserved SET domain responsible for methyltransferase activity, but exhibits distinct functional roles. While EZH2 is predominantly expressed in proliferating cells and drives H3K27me3 deposition during development and cancer progression, EZH1 is enriched in post-mitotic or differentiated cells, where it contributes to maintaining transcriptional repression and stabilizing cellular identity.
Recombinant EZH1 protein is engineered for in vitro studies to dissect its biochemical properties, substrate specificity, and interactions with PRC2 core components (e.g., SUZ12. EED, RBBP4). Its production typically involves heterologous expression systems (e.g., insect or mammalian cells) to ensure proper post-translational modifications and complex assembly. Research using recombinant EZH1 has revealed its context-dependent activity: it shows weaker methyltransferase efficiency compared to EZH2 but demonstrates unique redundancy or compensatory functions in EZH2-deficient conditions.
Studies highlight EZH1's dual role in both canonical PRC2-mediated gene repression and non-canonical processes, such as modulating RNA polymerase II dynamics. Dysregulation of EZH1 is implicated in developmental disorders, aging-related diseases, and malignancies, particularly in tissues with low proliferation rates. Recombinant EZH1 tools are pivotal for screening inhibitors targeting PRC2 in cancers and deciphering isoform-specific therapeutic strategies. Ongoing research aims to clarify its tissue-specific regulation and potential as a biomarker or drug target in precision medicine.
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