纯度 | >85%SDS-PAGE. |
种属 | Human |
靶点 | tTBK2 |
Uniprot No | Q6IQ55 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 18-287aa |
氨基酸序列 | KERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTF |
预测分子量 | 38.7 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与tTBK2(或相关重组TBK2蛋白)研究相关的文献摘要概括:
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1. **文献名称**: *"Expression and functional characterization of recombinant TBK2 kinase in innate immune signaling"*
**作者**: Smith A. et al. (2018)
**摘要**: 报道了通过大肠杆菌系统表达His标签的重组tTBK2蛋白,验证其激酶活性,并证明其在IRF3磷酸化和IFN-β信号通路中的调控作用。
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2. **文献名称**: *"Structural insights into TBK2 activation mechanism by cryo-EM analysis"*
**作者**: Chen L. & Wang H. (2020)
**摘要**: 解析了重组TBK2蛋白的冷冻电镜结构,揭示了其与适配蛋白TANK的结合模式及自磷酸化位点,为靶向TBK2的药物设计提供结构基础。
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3. **文献名称**: *"TBK2 kinase activity is essential for antiviral response in vitro"*
**作者**: Kimura T. et al. (2019)
**摘要**: 利用体外重组TBK2蛋白进行激酶活性测定,发现其通过磷酸化STING蛋白增强抗病毒信号通路,且活性缺失导致细胞对HSV-1感染的敏感性增加。
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4. **文献名称**: *"Development of a TBK2 recombinant protein-based assay for neurodegenerative disease research"*
**作者**: Rodriguez S. et al. (2021)
**摘要**: 开发了基于重组tTBK2蛋白的高通量筛选平台,用于评估激酶抑制剂对阿尔茨海默病模型细胞中tau蛋白异常磷酸化的影响。
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注:以上文献为示例性内容,实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献。
**Background of tTBK2 Recombinant Protein**
Tau tubulin kinase 2 (TTBK2) is a serine/threonine kinase encoded by the *TTBK2* gene, primarily known for its role in regulating cellular processes such as ciliogenesis, microtubule dynamics, and neurodegenerative pathways. It is enzymatically active in phosphorylating tau and tubulin proteins, which are critical for maintaining neuronal structure and function. Dysregulation of TTBK2 has been implicated in neurodegenerative disorders, including Alzheimer’s disease and spinocerebellar ataxia type 11 (SCA11), where mutations in *TTBK2* disrupt kinase activity or protein stability.
The recombinant tTBK2 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the kinase for research. This recombinant version often includes tags (e.g., His-tag) for efficient purification and detection. Researchers utilize tTBK2 to study its enzymatic mechanisms, substrate specificity, and interactions with partners like tau or components of the cilium assembly pathway.
Notably, tTBK2 is essential for initiating primary cilia disassembly by phosphorylating substrates that destabilize the ciliary base. Its role in tau phosphorylation also links it to tauopathies, making it a target for therapeutic strategies aimed at modulating kinase activity. Structural studies of recombinant tTBK2 have provided insights into its ATP-binding pocket and regulatory domains, facilitating drug discovery efforts.
Overall, tTBK2 recombinant protein serves as a vital tool for deciphering its biological functions, pathological contributions, and potential as a biomarker or drug target in neurodegenerative and ciliopathy-related diseases.
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