| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OIT3 |
| Uniprot No | Q8WWZ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-545aa |
| 氨基酸序列 | DPCSAYIS LNEPWRNTDH QLDESQGPPL CDNHVNGEWY HFTGMAGDAM PTFCIPENHC GTHAPVWLNG SHPLEGDGIV QRQACASFNG NCCLWNTTVE VKACPGGYYV YRLTKPSVCF HVYCGHFYDI CDEDCHGSCS DTSECTCAPG TVLGPDRQTC FDENECEQNN GGCSEICVNL KNSYRCECGV GRVLRSDGKT CEDVEGCHNN NGGCSHSCLG SEKGYQCECP RGLVLSEDNH TCQVPVLCKS NAIEVNIPRE LVGGLELFLT NTSCRGVSNG THVNILFSLK TCGTVVDVVN DKIVASNLVT GLPKQTPGSS GDFIIRTSKL LIPVTCEFPR LYTISEGYVP NLRNSPLEIM SRNHGIFPFT LEIFKDNEFE EPYREALPTL KLRDSLYFGI EPVVHVSGLE SLVESCFATP TSKIDEVLKY YLIRDGCVSD DSVKQYTSRD HLAKHFQVPV FKFVGKDHKE VFLHCRVLVC GVLDERSRCA QGCHRRMRRG AGGEDSAGLQ GQTLTGGPIR IDWED |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OIT3重组蛋白的假设参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *OIT3 Recombinant Protein Suppresses Viral Replication by Modulating Innate Immunity*
**作者**: Smith J. et al. (2018)
**摘要**: 本研究通过真核表达系统制备了OIT3重组蛋白，发现其能通过增强I型干扰素信号通路显著抑制RNA病毒(如流感病毒)的复制，提示其在抗病毒治疗中的潜力。
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2. **文献名称**: *Role of Recombinant OIT3 in Attenuating Hepatic Fibrosis via TGF-β Pathway*
**作者**: Zhang Y. et al. (2020)
**摘要**: 作者利用大肠杆菌表达并纯化OIT3重组蛋白，体外实验表明其通过靶向TGF-β/Smad信号通路减少肝星状细胞活化，为肝纤维化治疗提供新策略。
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3. **文献名称**: *OIT3 Recombinant Protein Alleviates Autoimmune Inflammation in Murine Models*
**作者**: Lee S. et al. (2021)
**摘要**: 研究显示，哺乳动物细胞表达的OIT3重组蛋白可降低Th17细胞分化，减轻实验性自身免疫性脑脊髓炎(EAE)模型炎症，提示其在自身免疫疾病中的应用价值。
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4. **文献名称**: *Recombinant OIT3 Inhibits Tumor Metastasis by Remodeling Extracellular Matrix*
**作者**: Chen L. et al. (2022)
**摘要**: 通过昆虫细胞表达系统获得高纯度OIT3蛋白，实验证实其通过抑制MMP-9活性减少肿瘤细胞侵袭，为癌症治疗提供新机制。
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**注意**：以上内容为示例性虚构，实际文献需在PubMed、Web of Science等平台以“OIT3 recombinant protein”或“OIT3 AND (expression OR function)”为关键词检索。若结果有限，建议扩展检索词或验证基因名称准确性(如是否涉及拼写变异或别名)。
**Background of OIT3 Recombinant Protein**
OIT3 (Oncoprotein Induced Transcript 3), also known as C22orf15 or CCDC3. is a protein-coding gene implicated in diverse cellular processes, including lipid metabolism, immune regulation, and viral infection responses. Initially identified through its transcriptional upregulation in response to oncogenic signals, OIT3 is characterized by a coiled-coil domain, suggesting roles in protein-protein interactions or structural organization. Studies link OIT3 to lipid droplet dynamics, where it may regulate lipid storage and metabolism in hepatocytes or adipocytes, potentially influencing metabolic disorders like obesity or fatty liver disease. Additionally, OIT3 has antiviral properties, particularly against hepatitis C virus (HCV), by interfering with viral replication or modulating host innate immunity.
Recombinant OIT3 protein is produced using biotechnological platforms (e.g., bacterial, mammalian, or insect cell systems) to enable functional studies. Its purification typically involves affinity tags (e.g., His-tag) and chromatographic techniques. Recombinant OIT3 serves as a critical tool for elucidating molecular mechanisms, screening therapeutic agents, or developing diagnostics. For instance, in metabolic research, it aids in studying lipid-associated pathways, while in virology, it helps dissect host-pathogen interactions.
Despite progress, OIT3’s precise molecular functions remain partially unresolved. Ongoing research focuses on its dual roles in metabolism and immunity, as well as its potential as a biomarker or therapeutic target. The development of recombinant OIT3 underscores its growing importance in bridging basic science with translational applications in metabolic and infectious diseases.
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