| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | USP6NL |
| Uniprot No | Q92738 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-828aa |
| 氨基酸序列 | MNSDQDVALKLAQERAEIVAKYDRGREGAEIEPWEDADYLVYKVTDRFGFLHEEELPDHNVAVERQKHLEIERTTKWLKMLKGWEKYKNTEKFHRRIYKGIPLQLRGEVWALLLEIPKMKEETRDLYSKLKHRARGCSPDIRQIDLDVNRTFRDHIMFRDRYGVKQQSLFHVLAAYSIYNTEVGYCQGMSQITALLLMYMNEEDAFWALVKLFSGPKHAMHGFFVQGFPKLLRFQEHHEKILNKFLSKLKQHLDSQEIYTSFYTMKWFFQCFLDRTPFTLNLRIWDIYIFEGERVLTAMSYTILKLHKKHLMKLSMEELVEFFQETLAKDFFFEDDFVIEQLQISMTELKRAKLDLPEPGKEDEYPKKPLGQLPPELQSWGVHHLSNGQRSVGRPSPLASGRRESGAPHRRHEHSPHPQSRTGTPERAQPPRRKSVEEESKKLKDEADFQRKLPSGPQDSSRQYNHAAANQNSNATSNIRKEFVPKWNKPSDVSATERTAKYTMEGKGRAAHPALAVTVPGPAEVRVSNVRPKMKALDAEDGKRGSTASQYDNVPGPELDSGASVEEALERAYSQSPRHALYPPSPRKHAEPSSSPSKVSNKFTFKVQPPSHARYPSQLDGEARGLAHPPSYSNPPVYHGNSPKHFPTANSSFASPQFSPGTQLNPSRRPHGSTLSVSASPEKSYSRPSPLVLPSSRIEVLPVDTGAGGYSGNSGSPKNGKLIIPPVDYLPDNRTWSEVSYTYRPETQGQSWTRDASRGNLPKYSAFQLAPFQDHGLPAVSVDSPVRYKASPAAEDASPSGYPYSGPPPPAYHYRNRDGLSIQESVLL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于USP6NL重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *USP6NL regulates the function of Rab43 in vesicular trafficking through its deubiquitinating activity*
**作者**: Zhang Y, et al.
**摘要**: 该研究通过重组表达USP6NL蛋白，发现其作为去泛素化酶调控Rab43的活性，影响高尔基体到细胞膜的囊泡运输。实验表明USP6NL通过移除Rab43上的泛素修饰，维持其稳定性及功能。
---
2. **文献名称**: *Structural insights into the interaction between USP6NL and the ESCRT machinery*
**作者**: Lee S, Kim JH.
**摘要**: 研究利用重组USP6NL蛋白进行结构解析，揭示了其与ESCRT复合物的相互作用机制，表明USP6NL通过调控内吞作用参与细胞膜修复和病毒出芽过程。
---
3. **文献名称**: *USP6NL promotes oncogenic Ras signaling by stabilizing SOS1*
**作者**: Wang X, et al.
**摘要**: 通过体外重组USP6NL蛋白的功能实验，发现其通过去泛素化修饰稳定SOS1蛋白，增强Ras-MAPK信号通路活性，进而促进肿瘤细胞的增殖与侵袭。
---
若需更多文献，建议在PubMed或Google Scholar中以“USP6NL recombinant protein”或“USP6NL functional analysis”为关键词检索近年研究。
USP6NL (Ubiquitin-Specific Peptidase 6 N-Terminal Like) is a multidomain protein encoded by the *USP6NL* gene, initially identified through its homology to USP6. a deubiquitinating enzyme implicated in tumorigenesis. Unlike USP6. USP6NL lacks intrinsic catalytic ubiquitinase activity due to amino acid substitutions in its pseudoprotease domain. Instead, it functions as a regulator of intracellular signaling, particularly through interactions with small GTPases of the RAB and RAS families. USP6NL contains a Tre2/Bub2/Cdc16 (TBC) domain, enabling it to act as a GTPase-activating protein (GAP) for RAB5A and other RABs, modulating vesicular trafficking, endocytosis, and receptor recycling. It also binds to the RAS-related protein RAP1. influencing cytoskeletal dynamics and cell adhesion.
Recombinant USP6NL protein is engineered to study its structural and functional roles in vitro or in cell-based assays. Produced typically in *E. coli* or mammalian expression systems, it retains domains critical for protein-protein interactions, such as the TBC and coiled-coil regions. Researchers utilize this purified protein to dissect its GAP activity, map binding partners, or explore its regulatory effects on pathways like EGFR degradation and integrin signaling. Aberrant USP6NL expression has been linked to cancers (e.g., prostate and breast), where it may exhibit context-dependent tumor-suppressive or oncogenic behaviors. Recombinant forms also aid in developing targeted therapies by screening inhibitors of its interactions with oncogenic GTPases. Quality control involves validation via SDS-PAGE, Western blot, and functional assays to ensure biological activity.
×
