| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDCCAG8 |
| Uniprot No | Q86SQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-713 aa |
| 活性数据 | MAKSPENSTL EEILGQYQRS LREHASRSIH QLTCALKEGD VTIGEDAPNL SFSTSVGNED ARTAWPELQQ SHAVNQLKDL LRQQADKESE VSPSRRRKMS PLRSLEHEET NMPTMHDLVH TINDQSQYIH HLEAEVKFCK EELSGMKNKI QVVVLENEGL QQQLKSQRQE ETLREQTLLD ASGNMHNSWI TTGEDSGVGE TSKRPFSHDN ADFGKAASAG EQLELEKLKL TYEEKCEIEE SQLKFLRNDL AEYQRTCEDL KEQLKHKEFL LAANTCNRVG GLCLKCAQHE AVLSQTHTNV HMQTIERLVK ERDDLMSALV SVRSSLADTQ QREASAYEQV KQVLQISEEA NFEKTKALIQ CDQLRKELER QAERLEKELA SQQEKRAIEK DMMKKEITKE REYMGSKMLI LSQNIAQLEA QVEKVTKEKI SAINQLEEIQ SQLASREMDV TKVCGEMRYQ LNKTNMEKDE AEKEHREFRA KTNRDLEIKD QEIEKLRIEL DESKQHLEQE QQKAALAREE CLRLTELLGE SEHQLHLTRQ EKDSIQQSFS KEAKAQALQA QQREQELTQK IQQMEAQHDK TENEQYLLLT SQNTFLTKLK EECCTLAKKL EQISQKTRSE IAQLSQEKRY TYDKLGKLQR RNEELEEQCV QHGRVHETMK QRLRQLDKHS QATAQQLVQL LSKQNQLLLE RQSLSEEVDR LRTQLPSMPQ SDC |
| 分子量 | 82.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人SDCCAG8蛋白的参考文献及其摘要总结：
1. **《SDCCAG8 interacts with RAB effector proteins and is required for ciliogenesis》**
- **作者**: Otto EA, et al. (2015)
- **摘要**: 本研究揭示了SDCCAG8与纤毛发生的关系，发现其通过与RAB效应蛋白相互作用调控囊泡运输，重组人SDCCAG8蛋白被用于验证其在纤毛基底体定位中的关键作用。突变导致肾囊肿和纤毛病表型。
2. **《The SDCCAG8 protein forms a conserved complex with oral-facial-digital syndrome proteins》**
- **作者**: Stiegler AL, et al. (2013)
- **摘要**: 利用重组表达技术证明SDCCAG8与OFD1等蛋白形成复合物，参与纤毛结构和功能调控。该复合物缺陷与多器官发育异常相关，为纤毛相关疾病的机制提供分子基础。
3. **《SDCCAG8 is a centrosome-centriole satellite protein required for ciliogenesis and mTOR signaling》**
- **作者**: Weihbrecht K, et al. (2018)
- **摘要**: 通过重组人SDCCAG8蛋白实验，发现其定位于中心体卫星结构，调控mTOR信号通路，影响纤毛形成。突变导致视网膜变性和肥胖症，提示其在代谢-纤毛轴中的双重功能。
4. **《A proteomic analysis reveals the role of SDCCAG8 in ciliary targeting of Bardet-Biedl syndrome proteins》**
- **作者**: Scheidecker S, et al. (2016)
- **摘要**: 利用重组SDCCAG8进行蛋白质组学分析，证实其作为衔接蛋白介导BBS复合物向纤毛的运输，突变导致蛋白错误定位，阐明了SDCCAG8在Bardet-Biedl综合征中的致病机制。
以上研究均通过重组蛋白技术探讨SDCCAG8的结构功能关系，聚焦于纤毛生物学及疾病关联机制。
SDCCAG8 (Serologically Defined Colon Cancer Antigen 8), also known as HSPC067 or CCCAP, is a protein encoded by the SDCCAG8 gene in humans. Initially identified as a potential tumor-associated antigen in colorectal cancer, it has since been linked to ciliary functions and cellular homeostasis. Structurally, SDCCAG8 contains coiled-coil domains and nuclear localization signals, suggesting roles in protein interactions and subcellular trafficking.
Mutations in SDCCAG8 are associated with ciliopathies, particularly Bardet-Biedl syndrome (BBS) and nephronophthisis-related disorders, manifesting as retinal degeneration, cystic kidneys, and developmental defects. The protein localizes to centrioles and ciliary basal bodies, playing a critical role in primary cilium assembly and maintenance. It interacts with proteins like OFD1 and TUBGCP4 to regulate ciliogenesis and cell cycle progression, influencing Hedgehog signaling and planar cell polarity pathways.
Recombinant human SDCCAG8 protein is produced via heterologous expression systems (e.g., E. coli, mammalian cells) for functional studies. It serves as a tool to investigate molecular mechanisms underlying ciliopathies, screen therapeutic agents, or analyze protein-protein interaction networks. Dysregulation of SDCCAG8 has also been implicated in cancer progression, possibly through mitotic errors or genomic instability. Ongoing research aims to clarify its dual roles in ciliary biology and tumorigenesis, with potential applications in diagnostics and targeted therapies.
×
