| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SDAD1 |
| Uniprot No | Q9NVU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-687 aa |
| 活性数据 | MSNRNNNKLP SNLPQLQNLI KRDPPAYIEE FLQQYNHYKS NVEIFKLQPN KPSKELAELV MFMAQISHCY PEYLSNFPQE VKDLLSCNHT VLDPDLRMTF CKALILLRNK NLINPSSLLE LFFELFRCHD KLLRKTLYTH IVTDIKNINA KHKNNKVNVV LQNFMYTMLR DSNATAAKMS LDVMIELYRR NIWNDAKTVN VITTACFSKV TKILVAALTF FLGKDEDEKQ DSDSESEDDG PTARDLLVQY ATGKKSSKNK KKLEKAMKVL KKQKKKKKPE VFNFSAIHLI HDPQDFAEKL LKQLECCKER FEVKMMLMNL ISRLVGIHEL FLFNFYPFLQ RFLQPHQREV TKILLFAAQA SHHLVPPEII QSLLMTVANN FVTDKNSGEV MTVGINAIKE ITARCPLAMT EELLQDLAQY KTHKDKNVMM SARTLIHLFR TLNPQMLQKK FRGKPTEASI EARVQEYGEL DAKDYIPGAE VLEVEKEENA ENDEDGWEST SLSEEEDADG EWIDVQHSSD EEQQEISKKL NSMPMEERKA KAAAISTSRV LTQEDFQKIR MAQMRKELDA APGKSQKRKY IEIDSDEEPR GELLSLRDIE RLHKKPKSDK ETRLATAMAG KTDRKEFVRK KTKTNPFSSS TNKEKKKQKN FMMMRYSQNV RSKNKRSFRE KQLALRDALL KKRKRMK |
| 分子量 | 79.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人SDAD1蛋白的文献参考示例(供学术格式参考)：
1. **文献名称**: *SDAD1 is required for the assembly of R-loop processing complexes and regulates transcription-coupled DNA repair*
**作者**: Chen et al.
**摘要**: 研究发现SDAD1作为RNA结合蛋白复合物的核心组分，参与调控R-loop动态平衡，缺失会导致转录-复制冲突增加，影响基因组稳定性，揭示了其在DNA损伤修复中的作用机制。
2. **文献名称**: *Expression and purification of recombinant human SDAD1 protein for structural studies*
**作者**: Smith et al.
**摘要**: 报道了通过大肠杆菌表达系统成功制备重组人SDAD1蛋白的优化方案，包括标签去除和纯化步骤，并通过X射线晶体学解析其N端结构域的原子结构，为功能研究提供基础。
3. **文献名称**: *SDAD1 deficiency suppresses neuroblastoma progression by destabilizing MYCN protein*
**作者**: Zhang et al.
**摘要**: 发现SDAD1在神经母细胞瘤中高表达，通过泛素化-蛋白酶体途径调控致癌因子MYCN的稳定性，靶向SDAD1可抑制肿瘤生长，提示其作为潜在治疗靶点。
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**备注**: 上述文献为模拟示例，实际文献需通过PubMed/Google Scholar检索获取。建议结合关键词“SDAD1”、“recombinant SDAD1 protein”、“SDA1 domain”查阅最新研究。
**Background of Recombinant Human SDAD1 Protein**
Recombinant human SDAD1 (SDA1 domain-containing protein 1) is a genetically engineered protein derived from the human *SDAD1* gene, which encodes a conserved eukaryotic protein implicated in transcriptional regulation and RNA metabolism. SDAD1 belongs to the SDA1 family, characterized by a conserved N-terminal SDA1 domain critical for interactions with RNA-binding proteins and participation in molecular complexes. Studies suggest its involvement in cell cycle regulation, stress response, and chromatin remodeling, potentially influencing cellular proliferation and apoptosis pathways.
The recombinant form of SDAD1 is typically expressed in heterologous systems (e.g., *E. coli* or mammalian cells) for functional studies, enabling high-purity production and scalability. Its recombinant production allows researchers to explore SDAD1's structural properties, interactome, and role in diseases such as cancer or neurodegenerative disorders. Notably, SDAD1 has been linked to modulating mRNA processing and stability, interacting with components of the exosome and transcription elongation complexes.
Despite progress, SDAD1's precise mechanisms remain partially unresolved, motivating ongoing research to elucidate its biological significance. Recombinant SDAD1 serves as a vital tool for deciphering its molecular functions and therapeutic potential, particularly in contexts where dysregulated RNA metabolism or transcriptional control contributes to pathology. This protein’s conserved nature across species further underscores its fundamental role in cellular processes.
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