关键信息
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基因名
RFT1
- 应用
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别名
RFT1; Protein RFT1 homolog
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96AA3
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表达区间
1-541 aa
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氨基酸序列
MGSQEVLGHAARLASSGLLLQVLFRLITFVLNAFILRFLSKEIVGVVNVRLTLLYSTTLFLAREAFRRACLSGGTQRDWSQTLNLLWLTVPLGVFWSLFLGWIWLQLLEVPDPNVVPHYATGVVLFGLSAVVELLGEPFWVLAQAHMFVKLKVIAESLSVILKSVLTAFLVLWLPHWGLYIFSLAQLFYTTVLVLCYVIYFTKLLGSPESTKLQTLPVSRITDLLPNITRNGAFINWKEAKLTWSFFKQSFLKQILTEGERYVMTFLNVLNFGDQGVYDIVNNLGSLVARLIFQPIEESFYIFFAKVLERGKDATLQKQEDVAVAAAVLESLLKLALLAGLTITVFGFAYSQLALDIYGGTMLSSGSGPVLLRSYCLYVLLLAINGVTECFTFAAMSKEEVDRYNFVMLALSSSFLVLSYLLTRWCGSVGFILANCFNMGIRITQSLCFIHRYYRRSPHRPLAGLHLSPVLLGTFALSGGVTAVSEVFLCCEQGWPARLAHIAVGAFCLGATLGTAFLTETKLIHFLRTQLGVPRRTDKMT
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分子量
86.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
RFT1 (Reduced Fundic Tension 1) is a protein implicated in various biological processes, particularly in the context of protein glycosylation and endoplasmic reticulum (ER) function. Research has shown that RFT1 plays a critical role in the transport of nucleotide-sugar glycosylation precursors into the ER, which is essential for proper glycoprotein synthesis. Mutations or dysfunctions in RFT1 can lead to various diseases, including congenital disorders of glycosylation. Investigating RFT1 recombinantly allows scientists to better understand its structure-function relationship, the mechanisms by which it facilitates sugar transport, and its broader implications in cellular biology. Moreover, RFT1's role in the ER stress response highlights its potential involvement in metabolic disorders, making it a focal point for therapeutic targeting. Recent studies have employed techniques such as CRISPR gene editing and proteomics to dissect the enzymatic pathways influenced by RFT1, further uncovering its significance in maintaining cellular homeostasis. Through these endeavors, RFT1 emerges not only as a vital component of the glycosylation machinery but also as a promising target for novel treatments aimed at addressing glycosylation-related diseases.
