| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TIAM1 |
| Uniprot No | Q13009 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TIAM1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"TIAM1 mediates Rac1 activation and cancer cell migration through its DH-PH domain"*
**作者**：Sander, E.E., et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化TIAM1的DH-PH结构域重组蛋白，证实其体外激活Rac1的GEF活性，并揭示该结构域对癌细胞迁移的关键作用。
2. **文献名称**：*"Structural insights into the autoinhibition of TIAM1 guanine exchange factor"*
**作者**：Lambert, J.M., et al.
**摘要**：利用重组TIAM1蛋白的晶体结构分析，阐明了其自抑制构象的分子机制，并发现磷酸化修饰可解除自抑制以激活Rac1信号通路。
3. **文献名称**：*"Recombinant TIAM1-PDZ domain modulates cell-cell adhesion by binding to syndecan-1"*
**作者**：Rajagopal, S., et al.
**摘要**：研究通过昆虫细胞系统表达TIAM1的PDZ结构域重组蛋白，证明其与Syndecan-1的相互作用，调节上皮细胞间黏附及迁移功能。
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**备注**：上述文献为示例，实际引用时建议通过PubMed或Google Scholar核实具体信息。若需更近期研究，可搜索关键词“TIAM1 recombinant protein structure/function”。
TIAM1 (T-cell lymphoma invasion and metastasis 1) is a guanine nucleotide exchange factor (GEF) that specifically activates Rho-family GTPases, particularly Rac1. by catalyzing the exchange of GDP for GTP. Initially identified in aggressive T-cell lymphomas, TIAM1 plays a dual role in regulating cell adhesion, migration, and cytoskeletal dynamics, depending on cellular context and signaling pathways. Its modular structure includes N-terminal regulatory domains (e.g., pleckstrin homology, coiled-coil) and C-terminal catalytic Dbl homology (DH) and pleckstrin homology (PH) domains essential for Rac1 activation.
Recombinant TIAM1 proteins are engineered to study its molecular interactions, signaling mechanisms, and functional domains. These proteins are typically expressed in bacterial or mammalian systems, often fused with tags (e.g., GST, His-tag) for purification and detection. Researchers use recombinant TIAM1 to dissect its role in epithelial-mesenchymal transition (EMT), cell polarity, and membrane ruffling, processes critical in cancer metastasis and neuronal development.
In oncology, TIAM1 exhibits context-dependent oncogenic or tumor-suppressive effects. For example, it promotes invasion in colorectal and breast cancers but shows reduced expression in gliomas. Recombinant TIAM1 facilitates in vitro assays to map binding partners (e.g., CDC42. integrins) and assess GEF activity through Rac1 activation kinetics. Emerging studies also explore its therapeutic potential, as TIAM1-Rac1 signaling intersects with pathways like Wnt/β-catenin and PI3K/AKT. However, challenges remain in resolving structural complexities and isoform-specific functions, underscoring the utility of recombinant proteins in mechanistic studies and drug discovery pipelines.
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