| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAT |
| Uniprot No | O43561-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-233aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMHCHRLPGSYDSTSSDSLYPRGIQFKRPHT VAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANS VASYENEEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTP GIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTE PAALSSQEAEEVEEEGAPDYENLQELN |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAT(Linker for Activation of T cells)重组蛋白的3-4条模拟参考文献示例(实际文献需通过学术数据库检索)：
1. **文献名称**：*"Structural and functional analysis of the LAT adaptor protein in T-cell signaling"*
**作者**：Samelson, L.E., et al.
**摘要**：该研究解析了LAT蛋白的胞内结构域及其在T细胞受体(TCR)信号传导中的关键作用，发现其酪氨酸磷酸化位点对下游信号分子(如PLCγ1和Grb2)的募集至关重要，重组LAT蛋白实验验证了其作为信号枢纽的功能。
2. **文献名称**：*"Recombinant LAT protein expression and purification for in vitro phosphorylation studies"*
**作者**：Zhang, W., et al.
**摘要**：报道了一种高效表达和纯化重组LAT蛋白的方法，利用大肠杆菌表达系统获得可溶性蛋白，并通过体外激酶实验证明LAT在ZAP-70激酶作用下的磷酸化动力学特性。
3. **文献名称**：*"LAT-mediated signaling in CD4+ T cell development and activation"*
**作者**：Acuto, O., et al.
**摘要**：通过基因工程构建LAT缺陷型T细胞系，并转染重组LAT突变体，揭示了LAP的特定结构域对T细胞发育、增殖及细胞因子分泌的调控机制。
4. **文献名称**：*"Dynamic clustering of LAT revealed by single-molecule imaging in living T cells"*
**作者**：Balagopalan, L., et al.
**摘要**：利用荧光标记的重组LAT蛋白结合活细胞成像技术，发现LAT在T细胞激活后形成动态微簇，这些簇的形成依赖于其跨膜结构域及与脂筏的相互作用，对信号放大至关重要。
**注意**：以上文献信息为示例性内容，实际引用需查询PubMed、Web of Science等数据库获取真实文献。
**Background of LAT Recombinant Protein**
The **Linker for Activation of T cells (LAT)** is a critical adaptor protein in T-cell receptor (TCR) signaling, playing a central role in mediating immune responses. LAT is a transmembrane protein with a short extracellular domain and a cytoplasmic tail containing multiple tyrosine residues. Upon TCR engagement, these tyrosine residues are phosphorylated, serving as docking sites for signaling molecules like PLC-γ1. Grb2. and Gads. This initiates downstream pathways, including calcium flux, MAPK activation, and NF-κB signaling, which drive T-cell proliferation, differentiation, and cytokine production.
**LAT recombinant proteins** are engineered versions of this protein, produced via genetic engineering in heterologous systems (e.g., *E. coli* or mammalian cells). These proteins retain functional domains but are often modified for research or therapeutic purposes, such as incorporating affinity tags (e.g., His-tag) for purification or mutating specific residues to study structure-function relationships. Recombinant LAT enables *in vitro* studies of TCR signaling mechanisms, immune synapse formation, and interactions with signaling partners.
Clinically, LAT is implicated in immune disorders. Mutations or dysregulation of LAT can lead to pathologies like severe combined immunodeficiency (SCID) or autoimmune diseases. Recombinant LAT proteins aid in developing targeted therapies, such as inhibitors of aberrant signaling in T-cell malignancies or tools to modulate immune responses in autoimmunity. Additionally, they are used in diagnostic assays to detect autoantibodies in diseases like lupus.
In summary, LAT recombinant proteins serve as vital tools for dissecting T-cell biology and advancing immunotherapeutic strategies, bridging basic research and clinical applications in immunology.
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