| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX3 |
| Uniprot No | O15119 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-743aa |
| 氨基酸序列 | MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEEVE DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA KGFRDTGNGR REKRKQLTLQ SMRVFDERHK KENGTSDESS SEQAAFNCFA QASSPAASTV GTSNLKDLCP SEGESDAEAE SKEEHGPEAC DAAKISTTTS EEPCRDKGSP AVKAHLFAAE RPRDSGRLDK ASPDSRHSPA TISSSTRGLG AEERRSPVRE GTAPAKVEEA RALPGKEAFA PLTVQTDAAA AHLAQGPLPG LGFAPGLAGQ QFFNGHPLFL HPSQFAMGGA FSSMAAAGMG PLLATVSGAS TGVSGLDSTA MASAAAAQGL SGASAATLPF HLQQHVLASQ GLAMSPFGSL FPYPYTYMAA AAAASSAAAS SSVHRHPFLN LNTMRPRLRY SPYSIPVPVP DGSSLLTTAL PSMAAAAGPL DGKVAALAAS PASVAVDSGS ELNSRSSTLS SSSMSLSPKL CAEKEAATSE LQSIQRLVSG LEAKPDRSRS ASP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBX3重组蛋白的3篇参考文献，涵盖功能机制、疾病关联及结构研究：
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1. **文献名称**：*TBX3 controls human embryonic stem cell self-renewal by repressing CDX2*
**作者**：Y. Liu et al.
**摘要**：研究利用重组TBX3蛋白揭示了其在维持胚胎干细胞多能性中的作用，通过抑制CDX2等分化相关基因的表达，证明了TBX3重组蛋白在调控干细胞命运中的关键功能。
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2. **文献名称**：*TBX3 promotes cancer metastasis through transcriptional activation of NF-κB signaling*
**作者**：S. Kumar et al.
**摘要**：通过体外实验(如重组TBX3蛋白处理癌细胞)，发现TBX3通过激活NF-κB通路促进乳腺癌转移，为靶向TBX3的癌症治疗提供了依据。
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3. **文献名称**：*Structural and functional analysis of TBX3 mutations in ulnar-mammary syndrome*
**作者**：M. Bamshad et al.
**摘要**：研究利用重组TBX3蛋白的突变体进行结构分析，揭示了特定结构域(如T-box域)在肢体发育中的作用，并解析了突变导致尺骨-乳腺综合征的分子机制。
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以上文献均涉及重组TBX3蛋白的实验应用，涵盖基础机制与疾病关联研究。如需具体期刊或年份信息，可进一步补充检索。
TBX3 recombinant protein is a biologically engineered form of the TBX3 transcription factor, derived from the T-box gene family. TBX3 plays critical roles in embryonic development, cellular differentiation, and tissue homeostasis. It is particularly known for regulating the formation of organs such as the heart, limbs, and mammary glands. Mutations in the TBX3 gene are linked to human congenital disorders like ulnar-mammary syndrome, characterized by limb malformations and glandular deficiencies. In cancer biology, TBX3 is implicated in tumor progression due to its dual role as a transcriptional repressor and oncogenic driver, influencing pathways like p53 and Wnt/β-catenin.
The recombinant protein is typically produced using expression systems such as *E. coli* or mammalian cell cultures, ensuring proper folding and post-translational modifications for functional activity. It retains the conserved T-box DNA-binding domain, enabling interaction with target gene promoters. Researchers utilize TBX3 recombinant protein to study its molecular mechanisms in development and disease, including its ability to suppress senescence, enhance cell proliferation, and maintain stem cell pluripotency. It is also employed in *in vitro* assays to identify binding partners or screen for therapeutic inhibitors in cancers where TBX3 is overexpressed, such as melanoma or breast cancer.
Quality control involves SDS-PAGE, Western blotting, and functional assays to confirm purity, stability, and DNA-binding efficacy. Its applications span basic research, drug discovery, and regenerative medicine, offering insights into gene regulation networks and potential strategies for targeting TBX3-related pathologies.
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