| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX21 |
| Uniprot No | Q9UL17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535aa |
| 氨基酸序列 | MGIVEPGCGDMLTGTEPMPGSDEGRAPGADPQHRYFYPEPGAQDADERRGGGSLGSPYPGGALVPAPPSRFLGAYAYPPRPQAAGFPGAGESFPPPADAEGYQPGEGYAAPDPRAGLYPGPREDYALPAGLEVSGKLRVALNNHLLWSKFNQHQTEMIITKQGRRMFPFLSFTVAGLEPTSHYRMFVDVVLVDQHHWRYQSGKWVQCGKAEGSMPGNRLYVHPDSPNTGAHWMRQEVSFGKLKLTNNKGASNNVTQMIVLQSLHKYQPRLHIVEVNDGEPEAACNASNTHIFTFQETQFIAVTAYQNAEITQLKIDNNPFAKGFRENFESMYTSVDTSIPSPPGPNCQFLGGDHYSPLLPNQYPVPSRFYPDLPGQAKDVVPQAYWLGAPRDHSYEAEFRAVSMKPAFLPSAPGPTMSYYRGQEVLAPGAGWPVAPQYPPKMGPASWFRPMRTLPMEPGPGGSEGRGPEDQGPPLVWTEIAPIRPESSDSGLGEGDSKRRRVSPYPSSGDSSSPAGAPSPFDKEAEGQFYNYFPN |
| 预测分子量 | 65.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Functional characterization of recombinant TBX21 in T lymphocyte differentiation"**
- Author: Szabo SJ et al.
- 摘要：研究利用重组TBX21蛋白验证其在Th1细胞分化中的核心调控作用，证实其通过结合IFN-γ启动子增强转录活性。
2. **"Purification and structural analysis of human TBX21 recombinant protein"**
- Author: Hwang ES et al.
- 摘要：报道TBX21重组蛋白的原核表达系统优化、纯化方法及晶体结构解析，揭示其DNA结合域的关键构象特征。
3. **"TBX21 recombinant protein modulates autoimmune inflammation in murine models"**
- Author: Lazarevic V et al.
- 摘要：通过体外递送重组TBX21蛋白，证明其可纠正Th1/Th2失衡，减轻实验性自身免疫性脑脊髓炎(EAE)模型症状。
4. **"High-throughput screening of TBX21-binding compounds using recombinant protein arrays"**
- Author: Tanaka S et al.
- 摘要：开发基于重组TBX21蛋白的微阵列技术，筛选出小分子抑制剂，为干预Th1相关疾病提供候选药物。
(注：以上文献信息为示例性概括，实际引用需核对具体论文数据)
TBX21. also known as T-bet (T-box transcription factor 21), is a critical regulator of immune cell differentiation and function. It belongs to the T-box family of transcription factors characterized by a conserved DNA-binding domain called the T-box. Discovered in 2000. TBX21 is primarily expressed in immune cells, including T helper 1 (Th1) cells, natural killer (NK) cells, and innate lymphoid cells, where it drives lineage-specific gene expression. Its most well-characterized role is in promoting Th1 cell differentiation by activating interferon-gamma (IFN-γ) production while suppressing Th2-associated cytokines like IL-4. This Th1/Th2 balance is essential for coordinating immune responses against intracellular pathogens and preventing allergic or autoimmune disorders.
Recombinant TBX21 protein refers to the engineered form of this transcription factor produced in vitro using expression systems such as *E. coli*, insect cells, or mammalian cell cultures. The protein typically retains the functional T-box domain required for DNA binding and interaction with transcriptional co-regulators. Researchers employ recombinant TBX21 to study its molecular mechanisms, including chromatin remodeling, promoter activation, and protein-protein interactions. It is also used to develop antibodies, screen small-molecule modulators, or engineer gene therapy vectors targeting Th1-mediated diseases.
Dysregulation of TBX21 is linked to autoimmune diseases (e.g., rheumatoid arthritis, multiple sclerosis), allergic inflammation, and cancer immunoediting. Recombinant TBX21 tools have advanced investigations into these conditions, offering insights for therapeutic strategies aimed at modulating immune polarization. Recent studies also explore its non-immune roles, such as in neuronal development and metabolic regulation, broadening its biomedical relevance.
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