| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXK |
| Uniprot No | P42681 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527aa |
| 氨基酸序列 | MILSSYNTIQ SVFCCCCCCS VQKRQMRTQI SLSTDEELPE KYTQRRRPWL SQLSNKKQSN TGRVQPSKRK PLPPLPPSEV AEEKIQVKAL YDFLPREPCN LALRRAEEYL ILEKYNPHWW KARDRLGNEG LIPSNYVTEN KITNLEIYEW YHRNITRNQA EHLLRQESKE GAFIVRDSRH LGSYTISVFM GARRSTEAAI KHYQIKKNDS GQWYVAERHA FQSIPELIWY HQHNAAGLMT RLRYPVGLMG SCLPATAGFS YEKWEIDPSE LAFIKEIGSG QFGVVHLGEW RSHIQVAIKA INEGSMSEED FIEEAKVMMK LSHSKLVQLY GVCIQRKPLY IVTEFMENGC LLNYLRENKG KLRKEMLLSV CQDICEGMEY LERNGYIHRD LAARNCLVSS TCIVKISDFG MTRYVLDDEY VSSFGAKFPI KWSPPEVFLF NKYSSKSDVW SFGVLMWEVF TEGKMPFENK SNLQVVEAIS EGFRLYRPHL APMSIYEVMY SCWHEKPEGR PTFAELLRAV TEIAETW |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TXK重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库验证)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant TXK Tyrosine Kinase in T-Cell Signaling*
**作者**：Smith A, et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组TXK蛋白，验证了其酪氨酸激酶活性。实验表明，重组TXK在体外能磷酸化下游底物STAT分子，并参与调控T细胞受体(TCR)介导的NF-κB信号通路，提示其在免疫应答中的关键作用。
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2. **文献名称**：*Structural Insights into TXK Kinase Domain via Recombinant Protein Crystallography*
**作者**：Chen L, et al.
**摘要**：通过重组TXK激酶结构域的表达与结晶，解析了其高分辨率三维结构。分析发现，TXK的ATP结合口袋具有独特的构象，为设计靶向T细胞相关疾病的特异性抑制剂提供了结构基础。
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3. **文献名称**：*Recombinant TXK Protein as a Therapeutic Target in Autoimmune Disorders*
**作者**：Wang Y, et al.
**摘要**：研究利用重组TXK蛋白筛选小分子化合物库，发现一种新型抑制剂可有效阻断TXK介导的炎症因子释放。动物模型实验显示，该抑制剂显著减轻了类风湿性关节炎症状，表明TXK是潜在的治疗靶点。
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4. **文献名称**：*Optimization of TXK Recombinant Protein Production in Mammalian Cells*
**作者**：Johnson R, et al.
**摘要**：通过哺乳动物细胞表达系统优化重组TXK的分泌表达条件，提高了蛋白产量与稳定性。纯化的重组蛋白在功能上接近天然TXK，适用于高通量药物筛选及抗体开发。
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**注**：以上内容为模拟示例，实际文献需以具体数据库检索结果为准。建议使用关键词“TXK recombinant protein”、“TXK tyrosine kinase”在PubMed或Google Scholar中查找最新研究。
TXK recombinant protein is derived from the TXK gene, which encodes a non-receptor tyrosine kinase belonging to the TEC kinase family. Primarily expressed in T lymphocytes and natural killer (NK) cells, TXK plays a critical role in intracellular signaling pathways regulating immune responses. It is involved in T-cell receptor (TCR) signaling, cytokine production (e.g., IL-2), and cell proliferation/differentiation. Structurally, TXK contains a pleckstrin homology (PH) domain, a Tec homology (TH) domain, and a kinase domain, enabling interactions with downstream signaling molecules like PLCγ1 and Vav1.
The recombinant form of TXK is typically produced in vitro using expression systems such as *E. coli* or mammalian cells. This engineered protein retains functional domains essential for studying its enzymatic activity, substrate binding, or interactions in immune regulation. Researchers utilize TXK recombinant protein to investigate its role in autoimmune diseases (e.g., rheumatoid arthritis, multiple sclerosis) and cancers, where dysregulated TXK expression correlates with pathological T-cell activation or tumor evasion.
In drug discovery, TXK is explored as a therapeutic target for immunomodulation. Inhibitors targeting its kinase activity may suppress aberrant T-cell responses in autoimmune conditions. Additionally, TXK recombinant protein serves as a tool for antibody development, structural studies, and high-throughput screening assays. Its applications extend to elucidating mechanisms of TCR signaling crosstalk with costimulatory molecules like CD28.
Ongoing research focuses on TXK’s dual role in pro-inflammatory and regulatory T-cell functions, aiming to balance therapeutic efficacy and safety. As a biomarker or therapeutic candidate, TXK recombinant protein remains pivotal in advancing immunology and precision medicine.
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