| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TWINL |
| Uniprot No | Q96RR1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 32-684aa |
| 氨基酸序列 | APGPPRRRYRKETLQALDMPVLPVTATEIRQYLRGHGIPFQDGHSCLRALSPFAESSQLKGQTGVTTSFSLFIDKTTGHFLCMTSLAEGSWEDFQASVEGRGDGAREGFLLSKAPEFEDSEEVRRIWNRAIPLWELPDQEEVQLADTMFGLTKVTDDTLKRFSVRYLRPARSLVFPWFSPGGSGLRGLKLLEAKCQGDGVSYEETTIPRPSAYHNLFGLPLISRRDAEVVLTSRELDSLALNQSTGLPTLTLPRGTTCLPPALLPYLEQFRRIVFWLGDDLRSWEAAKLFARKLNPKRCFLVRPGDQQPRPLEALNGGFNLSRILRTALPAWHKSIVSFRQLREEVLGELSNVEQAAGLRWSRFPDLNRILKGHRKGELTVFTGPTGSGKTTFISEYALDLCSQGVNTLWGSFEISNVRLARVMLTQFAEGRLEDQLDKYDHWADRFEDLPLYFMTFHGQQSIRTVIDTMQHAVYVYDICHVIIDNLQFMMGHEQLSTDRIAAQDYIIGVFRKFATDNNCHVTLVIHPRKEDDDKELQTASIFGSAKASQEADNVLILQDRKLVTGPGKRYLQVSKNRFDGDVGVFPLEFNKNSLTFSIPPKNKARLKKIKDDTGPVAKKPSSGKKGATTQNSEICSGQAPTPDQPDTSKRSK |
| 预测分子量 | 75.5kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TWINL重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供格式参考)：
---
1. **文献名称**：Expression and Functional Characterization of Recombinant TWINL Protein in Escherichia coli
**作者**：Li, X. et al.
**摘要**：本研究成功构建了TWINL重组蛋白的原核表达系统，通过大肠杆菌实现了高效可溶性表达。纯化后的蛋白在体外验证了其ATP酶活性，为后续功能研究奠定基础。
2. **文献名称**：Structural Insights into TWINL Protein-DNA Interaction by Cryo-EM Analysis
**作者**：Wang, Y. et al.
**摘要**：利用冷冻电镜技术解析了TWINL重组蛋白与DNA复合物的三维结构，揭示了其保守结构域对核酸结合的关键作用，为理解其在DNA修复中的分子机制提供依据。
3. **文献名称**：TWINL Recombinant Protein Enhances Neuronal Regeneration in a Zebrafish Model
**作者**：Garcia, R. et al.
**摘要**：在斑马鱼模型中验证了TWINL重组蛋白对神经元再生的促进作用，发现其通过激活Wnt/β-catenin信号通路促进轴突生长，具有潜在治疗神经退行性疾病的潜力。
---
建议通过PubMed或Web of Science以“TWINL recombinant protein”为关键词检索真实文献。若研究领域较新，可能需要结合相关蛋白别名(如TWINKLE或TWNK)扩大检索范围。
TWINL (TWIN-like) recombinant protein is a synthetic protein construct inspired by the structural and functional attributes of the TWINKLE protein, a mitochondrial DNA helicase critical for mtDNA replication and maintenance. TWINKLE, encoded by the *TWNK* gene in humans, plays a central role in mitochondrial genome stability by unwinding duplex DNA during replication. Mutations in *TWNK* are linked to mitochondrial disorders such as progressive external ophthalmoplegia (PEO) and infantile-onset spinocerebellar ataxia (IOSCA), underscoring its biological significance.
The development of TWINL recombinant protein emerged from the need to study TWINKLE’s molecular mechanisms in vitro and explore therapeutic interventions for mitochondrial diseases. Recombinant TWINL is typically engineered using bacterial or eukaryotic expression systems, enabling large-scale production for biochemical assays. It retains key functional domains of TWINKLE, including helicase activity and nucleotide-binding regions, while incorporating modifications for enhanced stability or solubility. Researchers utilize TWINL to investigate helicase kinetics, protein-DNA interactions, and the impact of disease-associated mutations on enzymatic function. Additionally, it serves as a tool for drug screening to identify compounds that modulate mtDNA replication efficiency. Recent studies also explore its potential in gene therapy or mitochondrial-targeted treatments. By providing a controllable and reproducible model, TWINL recombinant protein bridges gaps in understanding mitochondrial dysfunction and accelerates translational research in aging-related and genetic metabolic disorders.
×
