| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRAF4 |
| Uniprot No | Q9BUZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MPGFDYKFLE KPKRRLLCPL CGKPMREPVQ VSTCGHRFCD TCLQEFLSEG VFKCPEDQLP LDYAKIYPDP ELEVQVLGLP IRCIHSEEGC RWSGPLRHLQ GHLNTCSFNV IPCPNRCPMK LSRRDLPAHL QHDCPKRRLK CEFCGCDFSG EAYESHEGMC PQESVYCENK CGARMMRRLL AQHATSECPK RTQPCTYCTK EFVFDTIQSH QYQCPRLPVA CPNQCGVGTV AREDLPGHLK DSCNTALVLC PFKDSGCKHR CPKLAMARHV EESVKPHLAM MCALVSRQRQ ELQELRRELE ELSVGSDGVL IWKIGSYGRR LQEAKAKPNL ECFSPAFYTH KYGYKLQVSA FLNGNGSGEG THLSLYIRVL PGAFDNLLEW PFARRVTFSL LDQSDPGLAK PQHVTETFHP DPNWKNFQKP GTWRGSLDES SLGFGYPKFI SHQDIRKRNY VRDDAVFIRA AVELPRKILS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRAF4重组蛋白的3篇参考文献示例(注：文献为示例性概括，非真实存在)：
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1. **文献名称**: *"Expression and Purification of Recombinant TRAF4 for Structural Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌系统中高效表达和纯化重组TRAF4蛋白的方法。通过优化表达条件和亲和层析技术，获得了高纯度蛋白，并验证了其二级结构完整性，为后续的晶体学研究和功能分析奠定了基础。
2. **文献名称**: *"TRAF4 Recombinant Protein Interacts with TGF-β Receptor in Signaling Pathway Regulation"*
**作者**: Johnson B, et al.
**摘要**: 利用哺乳动物细胞表达系统制备了重组TRAF4蛋白，并通过体外结合实验证实其与TGF-β受体的直接相互作用。研究揭示了TRAF4在TGF-β信号通路中的调控机制，为癌症相关信号传导研究提供了新视角。
3. **文献名称**: *"Structural Insights into TRAF4 Ubiquitination Function via Recombinant Protein Assays"*
**作者**: Chen L, et al.
**摘要**: 通过重组TRAF4蛋白的体外泛素化实验，鉴定了其E3泛素连接酶活性依赖的关键结构域。冷冻电镜解析的TRAF4三维结构揭示了其与其他TRAF家族成员的差异，解释了其在特定癌症中的异常激活机制。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“TRAF4 recombinant protein”或结合具体研究方向筛选。
**Background of TRAF4 Recombinant Protein**
TRAF4 (TNF receptor-associated factor 4) is a member of the TRAF protein family, which plays critical roles in regulating cellular signaling pathways, particularly those mediated by tumor necrosis factor (TNF) receptors, Toll-like receptors (TLRs), and cytokine receptors. Unlike other TRAF proteins (e.g., TRAF2. TRAF6), TRAF4 exhibits unique features in subcellular localization, binding partners, and biological functions. It is involved in diverse processes, including immune response modulation, stress signaling, embryonic development, and tissue homeostasis. Structurally, TRAF4 contains a conserved C-terminal TRAF domain responsible for protein-protein interactions, a central coiled-coil region, and an N-terminal RING finger domain with potential E3 ubiquitin ligase activity.
Recombinant TRAF4 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via expression systems like *E. coli* or mammalian cells, the purified protein retains functional domains, enabling researchers to analyze its interactions with signaling adaptors (e.g., TNF receptors, kinases), ubiquitination activity, and role in pathways like NF-κB or MAPK. TRAF4 dysregulation has been linked to cancers (e.g., breast, prostate), developmental defects, and inflammatory diseases, making its recombinant form valuable for drug discovery and mechanistic studies.
Studies using recombinant TRAF4 have revealed its context-dependent roles—acting as an oncogene in certain cancers while suppressing metastasis in others. Its ability to shuttle between cytoplasm and nucleus further underscores its regulatory complexity. Overall, recombinant TRAF4 serves as a key tool for unraveling its dualistic functions and therapeutic potential.
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