| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FYN |
| Uniprot No | P06241 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-537aa |
| 氨基酸序列 | GCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYN NFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDL SFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYF GKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVK HYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMP RLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTL KPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLL DFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNG LICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWS FGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIH CWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FYN重组蛋白的3篇参考文献示例(注：文献为虚构，仅供参考格式及内容方向)：
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1. **文献名称**: *"High-Yield Expression and Purification of Active FYN Kinase in a Bacterial System"*
**作者**: Smith J, Brown K, Zhang L
**摘要**: 本研究报道了在大肠杆菌中高效表达并纯化具有生物活性的FYN酪氨酸激酶的方法。通过密码子优化和温度诱导策略，成功获得可溶性重组FYN蛋白，并验证其通过自磷酸化及底物磷酸化活性的功能，为体外激酶研究提供可靠工具。
2. **文献名称**: *"Structural Analysis of Recombinant FYN SH2 Domain Reveals Substrate Binding Specificity"*
**作者**: Lee S, García-Ruiz E, Patel R
**摘要**: 利用重组表达的FYN SH2结构域，结合X射线晶体学技术解析其三维结构，揭示了该结构域与磷酸化肽段相互作用的分子机制，为设计靶向FYN信号通路的抑制剂奠定结构基础。
3. **文献名称**: *"Functional Role of Recombinant FYN in Neuronal Differentiation In Vitro"*
**作者**: Wang H, Müller T, Costa M
**摘要**: 通过哺乳动物细胞表达系统制备重组FYN蛋白，研究其在神经元分化中的作用。实验表明FYN通过调控MAPK信号通路促进神经突生长，提示其在神经发育疾病中的潜在治疗价值。
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**建议**：实际文献需通过PubMed、Web of Science等平台，以关键词“FYN recombinant protein expression/purification/function”检索，并筛选近年高影响力期刊文章(如*Journal of Biological Chemistry*、*Protein Expression and Purification*等)。
FYN recombinant protein is a biologically engineered form of the FYN tyrosine kinase, a member of the Src family of non-receptor tyrosine kinases. FYN plays a critical role in intracellular signaling, particularly in immune response regulation, neuronal development, and cell adhesion. It contains SH2 and SH3 domains that mediate protein-protein interactions, facilitating signal transduction pathways. FYN is involved in T-cell receptor signaling, integrin-mediated adhesion, and neural synapse formation, with dysregulation linked to cancers, neurodegenerative disorders (e.g., Alzheimer’s disease), and autoimmune diseases.
Recombinant FYN is produced using expression systems like *E. coli* or mammalian cell lines (e.g., HEK293), ensuring proper post-translational modifications for functional studies. Researchers use it to investigate FYN’s kinase activity, interactions with downstream effectors (e.g., PI3K, MAPK pathways), and roles in pathological contexts. Its applications include *in vitro* kinase assays, drug screening for FYN inhibitors, and mechanistic studies in cancer immunotherapy or neurological research. By providing a purified, active form of FYN, this recombinant tool accelerates the exploration of therapeutic targets and disease mechanisms tied to FYN signaling.
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