| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HES1 |
| Uniprot No | Q14469 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-142aa |
| 氨基酸序列 | KSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTV KHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEV RTRLLGH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HES1重组蛋白的参考文献及其摘要概括：
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1. **文献名称**：*"Structural and functional analysis of the human HES1 promoter and its regulation by recombinant HES1 protein"*
**作者**：Jarriault, S.; Brou, C.; Logeat, F.; et al.
**摘要**：本研究通过表达并纯化重组HES1蛋白，分析了其对自身启动子的转录抑制机制，发现HES1通过结合特定DNA序列(N-box)抑制转录活性，揭示了其自我调控的负反馈环路。
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2. **文献名称**：*"Recombinant HES1 protein regulates neural stem cell differentiation through interaction with EZH2"*
**作者**：Kondo, T.; Raff, M.
**摘要**：研究利用大肠杆菌表达系统制备重组HES1蛋白，证明其通过与表观调控因子EZH2结合，抑制神经干细胞向神经元分化的关键基因，维持干细胞多能性。
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3. **文献名称**：*"Purification and functional characterization of the HES1 recombinant protein in pancreatic cancer cells"*
**作者**：Sang, L.; Roberts, J.M.; Collisson, E.A.
**摘要**：通过昆虫细胞表达系统获得高纯度HES1重组蛋白，发现其通过抑制Notch通路下游靶点(如Hey1)调控胰腺癌细胞增殖和上皮-间质转化(EMT)。
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如果需要具体文献来源或更多细节，可进一步提供DOI或数据库检索关键词。
HES1 (Hairy and Enhancer of Split 1) is a member of the HES family of basic helix-loop-helix (bHLH) transcriptional repressors, primarily known for its role in the Notch signaling pathway. It functions as a downstream effector of Notch signaling, regulating processes such as neurogenesis, stem cell maintenance, and cellular differentiation. HES1 binds to N-box or E-box DNA sequences in target gene promoters to suppress transcription, often forming negative feedback loops to modulate its own expression and maintain dynamic oscillations critical for cell fate decisions.
Recombinant HES1 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells) to ensure proper folding and post-translational modifications. The protein is purified using affinity tags (e.g., His-tag) and validated via SDS-PAGE, Western blot, or functional assays. Its applications span *in vitro* studies, including dissecting Notch pathway mechanisms, stem cell differentiation protocols, and cancer research, where HES1 dysregulation is linked to tumor progression and therapy resistance.
Researchers also utilize recombinant HES1 to explore its interactions with co-repressors (e.g., Groucho/TLE proteins) or competing bHLH activators (e.g., ASCL1). Its oscillatory expression pattern, observed in developmental processes, is studied using live-cell imaging or synthetic reporter systems. Beyond basic science, HES1 recombinant protein holds potential in drug screening for diseases like glioblastoma or T-cell leukemia, where Notch-HES1 signaling is pathologically active. However, challenges remain in mimicking its native dynamics *in vitro* due to its short half-life and context-dependent activity.
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