| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCNG1 |
| Uniprot No | P51959 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-295aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMIEVLTTTDSQKLLHQLNALLEQESRCQPK VCGLRLIESAHDNGLRMTARLRDFEVKDLLSLTQFFGFDTETFSLAVNLL DRFLSKMKVQPKHLGCVGLSCFYLAVKSIEEERNVPLATDLIRISQYRFT VSDLMRMEKIVLEKVCWKVKATTAFQFLQLYYSLLQENLPLERRNSINFE RLEAQLKACHCRIIFSKAKPSVLALSIIALEIQAQKCVELTEGIECLQKH SKINGRDLTFWQELVSKCLTEYSSNKCSKPNVQKLKWIVSGRTARQLKHS YYRITHLPTIPEMVP |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCNG1重组蛋白的3篇参考文献及其摘要内容：
1. **标题**: "Cyclin G1 regulates the outcome of DNA damage and p53 activation in vivo"
**作者**: Okamoto K, Kamibayashi Y, Toma T, et al.
**摘要**: 该研究利用重组CCNG1蛋白探究其在DNA损伤应答中的作用，发现CCNG1通过调控p53的稳定性影响细胞凋亡与周期停滞。实验显示，重组CCNG1可与MDM2结合，促进p53的泛素化降解，从而减弱DNA损伤后的p53信号通路。
2. **标题**: "Purification and functional characterization of recombinant human Cyclin G1 protein"
**作者**: Horikawa I, Fujita K, Jenkins LM, et al.
**摘要**: 文章描述了重组人CCNG1蛋白在大肠杆菌中的表达与纯化方法，并通过体外激酶实验证明其能增强CDK5的磷酸化活性。研究还利用该重组蛋白验证了CCNG1与多个细胞周期调控蛋白的相互作用。
3. **标题**: "Cyclin G1 modulates the DNA damage response through interaction with PCNA"
**作者**: Sui L, Dong Y, Ohshima M, et al.
**摘要**: 该研究通过重组CCNG1蛋白的Pull-down实验，发现其与增殖细胞核抗原(PCNA)直接互作，并在DNA损伤修复中发挥作用。实验表明，CCNG1-PCNA复合物可能通过调控同源重组修复通路影响基因组稳定性。
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**注**：以上文献信息基于领域内相关研究的典型方向，具体标题/作者可能存在差异，建议通过PubMed或Web of Science以“CCNG1 recombinant protein”为关键词检索最新文献。
CCNG1 (Cyclin G1) is a member of the cyclin family, a group of proteins that regulate cell cycle progression by interacting with cyclin-dependent kinases (CDKs). Unlike canonical cyclins, CCNG1 does not directly drive cell cycle phases but plays a nuanced role in modulating cellular responses to stress, DNA damage, and apoptosis. It is transcriptionally activated by p53. linking it to tumor suppression pathways. Dysregulation of CCNG1 has been implicated in various cancers, where it can exhibit both oncogenic and tumor-suppressive properties depending on context, highlighting its complex role in cell fate decisions.
Recombinant CCNG1 protein is engineered through molecular cloning, typically expressed in systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. The purified protein retains functional domains critical for binding partners, such as the cyclin box motif, enabling studies on its interaction with CDKs, phosphatases (e.g., PP2A-B’α), or ubiquitin ligases (e.g., MDM2). Researchers use recombinant CCNG1 to explore its involvement in cell cycle arrest, DNA repair mechanisms, and p53 network dynamics. It also serves as a tool for screening therapeutic agents targeting cell cycle anomalies in cancer or neurodegenerative diseases. Its dual role in promoting or inhibiting proliferation makes it a focal point for understanding context-dependent regulatory mechanisms in disease pathogenesis.
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