| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Slit1 |
| Uniprot No | O75093 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于Slit1重组蛋白的参考文献概览：
1. **《Slit1-mediated chemorepulsion guides cranial motor axon projections in the developing chick embryo》**
- 作者: M. M. Tam et al.
- 摘要: 研究利用重组Slit1蛋白证明其在鸡胚运动神经元轴突导向中的化学排斥作用，揭示其通过Robo受体调控神经回路形成的分子机制。
2. **《Recombinant Slit1 suppresses glioblastoma cell migration via modulation of actin dynamics》**
- 作者: K. L. Huang & J. R. Park
- 摘要: 发现重组Slit1蛋白可抑制胶质母细胞瘤细胞迁移，通过下调Cdc42/Rac1信号通路影响细胞骨架重组，提示其潜在的抗肿瘤转移应用价值。
3. **《Functional characterization of N-terminal truncated Slit1 variants in angiogenesis》**
- 作者: S. Q. Wu et al.
- 摘要: 通过杆状病毒系统表达不同截短型Slit1重组蛋白，证实其N端结构域对血管内皮细胞趋化性和血管生成的抑制作用，为抗血管治疗提供新靶点。
注：以上文献信息为模拟示例，实际引用前请核对PubMed等数据库获取真实文献。建议补充检索关键词：Slit1 recombinant + axon guidance/cancer/angiogenesis。
Slit1 is a secreted glycoprotein belonging to the Slit family of extracellular matrix-associated proteins, primarily known for their role in axon guidance and cellular migration during neural development. Initially identified in *Drosophila*, Slit proteins (Slit1. Slit2. Slit3 in mammals) interact with Roundabout (Robo) receptors to regulate repulsive signaling pathways that guide growing axons and influence neuronal cell migration. Slit1 is predominantly expressed in the central nervous system (CNS), particularly in the floor plate of the neural tube, and plays critical roles in establishing neural circuit connectivity by steering axon growth cones and preventing midline recrossing.
Recombinant Slit1 protein is generated through biotechnological methods, often using mammalian expression systems (e.g., HEK293 or CHO cells) or bacterial systems to produce purified, bioactive forms. Its functional domain structure includes leucine-rich repeats (LRR), epidermal growth factor (EGF)-like domains, and a cysteine-rich C-terminal region, which are essential for receptor binding and signal modulation. Researchers utilize recombinant Slit1 to study its chemorepellent activity, role in neuronal patterning, and involvement in non-neuronal processes such as angiogenesis, immune cell migration, and cancer metastasis. Notably, Slit1-Robo signaling has been implicated in inhibiting cell migration by modulating cytoskeletal dynamics via Rho GTPases.
In therapeutic contexts, recombinant Slit1 is explored for neuroregeneration strategies, anti-angiogenic therapies, and as a potential inhibitor of tumor invasion. Its ability to regulate cellular motility and axonal navigation makes it a valuable tool for both developmental biology research and translational applications.
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