| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SESN2 |
| Uniprot No | P58004 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-480aa |
| 氨基酸序列 | IVADSECRAELKDYLRFAPGGVGDSGPGEEQRESRARRGPRGPSAFIPVE EVLREGAESLEQHLGLEALMSSGRVDNLAVVMGLHPDYFTSFWRLHYLLL HTDGPLASSWRHYIAIMAAARHQCSYLVGSHMAEFLQTGGDPEWLLGLHR APEKLRKLSEINKLLAHRPWLITKEHIQALLKTGEHTWSLAELIQALVLL THCHSLSSFVFGCGILPEGDADGSPAPQAPTPPSEQSSPPSRDPLNNSGG FESARDVEALMERMQQLQESLLRDEGTSQEEMESRFELEKSESLLVTPSA DILEPSPHPDMLCFVEDPTFGYEDFTRRGAQAPPTFRAQDYTWEDHGYSL IQRLYPEGGQLLDEKFQAAYSLTYNTIAMHSGVDTSVLRRAIWNYIHCVF GIRYDDYDYGEVNQLLERNLKVYIKTVACYPEKTTRRMYNLFWRHFRHSE KVHVNLLLLEARMQAALLYALRAITRYMT |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SESN2(Sestrin2)重组蛋白的模拟参考文献示例，供参考(建议通过学术数据库验证真实文献)：
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1. **文献名称**: *"Recombinant Sestrin2 Protein Attenuates Oxidative Stress via Nrf2 Activation in Cellular Models"*
**作者**: Kim, J.H.; Lee, S.
**摘要**: 本研究报道了通过大肠杆菌系统成功表达并纯化重组SESN2蛋白。实验表明，该蛋白通过激活Nrf2-ARE通路显著降低H2O2诱导的氧化损伤，提示其在抗氧化治疗中的潜在应用。
2. **文献名称**: *"Sestrin2 Recombinant Protein Suppresses mTORC1 Signaling and Protects against Diet-Induced Obesity in Mice"*
**作者**: Peng, M.; et al.
**摘要**: 作者利用哺乳动物细胞表达系统制备重组SESN2蛋白，发现其通过抑制mTORC1通路改善小鼠代谢紊乱，为肥胖相关疾病的干预提供了实验依据。
3. **文献名称**: *"Structural and Functional Characterization of Recombinant Human Sestrin2"*
**作者**: Chen, X.; Wang, Q.
**摘要**: 通过X射线晶体学解析重组人源SESN2蛋白的三维结构，并验证其与AMPK的结合能力，揭示了SESN2在能量应激响应中的分子机制。
4. **文献名称**: *"Recombinant SESN2 Enhances Autophagy and Reduces Ischemia-Reperfusion Injury in Cardiac Cells"*
**作者**: Zhang, Y.; Liu, T.
**摘要**: 研究证明，外源性重组SESN2蛋白可通过诱导自噬减轻心肌细胞的缺血再灌注损伤，为心血管疾病治疗提供了新思路。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：Sestrin2. recombinant protein, oxidative stress, mTOR, autophagy)。
**Background of SESN2 Recombinant Protein**
SESN2 (Sestrin 2) is a stress-inducible protein belonging to the sestrin family, which plays a critical role in cellular responses to oxidative stress, DNA damage, and nutrient deprivation. It is encoded by the *SESN2* gene and functions as a conserved antioxidant protein, regulating redox homeostasis by activating the NRF2 pathway and suppressing reactive oxygen species (ROS) accumulation. SESN2 also interacts with key metabolic regulators, including mTORC1 (mechanistic target of rapamycin complex 1), acting as a negative modulator to inhibit anabolic processes under stress conditions, thereby promoting autophagy and metabolic adaptation.
Recombinant SESN2 protein is produced through genetic engineering, typically using bacterial or mammalian expression systems, to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native SESN2. enabling its use in studying molecular mechanisms of oxidative stress, aging, and metabolic diseases. Research highlights its therapeutic potential in conditions like diabetes, neurodegenerative disorders, and cancer, where dysregulated ROS signaling or mTOR pathways are implicated.
Recent studies also suggest SESN2's role in modulating AMP-activated protein kinase (AMPK) activity, further linking it to energy sensing and longevity. Its recombinant form is widely utilized in *in vitro* and *in vivo* models to explore these pathways, offering insights into drug development targeting stress-related pathologies. Additionally, SESN2 recombinant protein serves as a tool for structural analysis, antibody production, and biomarker discovery, underscoring its versatility in both basic and applied biomedical research.
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