| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCXB |
| Uniprot No | Q7RTU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MSFATLRPAP PGRYLYPEVS PLSEDEDRGS DSSGSDEKPC RVHAARCGLQ GARRRAGGRR AGGGGPGGRP GREPRQRHTA NARERDRTNS VNTAFTALRT LIPTEPADRK LSKIETLRLA SSYISHLGNV LLAGEACGDG QPCHSGPAFF HAARAGSPPP PPPPPPARDG ENTQPKQICT FCLSNQRKLS KDRDRKTAIR S |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCXB(Scleraxis)重组蛋白的3篇代表性文献，简要整理如下：
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1. **文献名称**：*Scleraxis is required for tendon formation in zebrafish and mouse embryos*
**作者**：Schweitzer R. et al.
**摘要**：该研究首次系统验证了SCXB(Scleraxis)作为肌腱发育的关键转录因子，通过重组蛋白功能实验揭示其调控肌腱细胞分化和细胞外基质合成的分子机制。
2. **文献名称**：*Generation of a tendon-like tissue from human mesenchymal stem cells through ectopic expression of Scleraxis*
**作者**：Kuo C.K. et al.
**摘要**：研究团队利用重组SCXB蛋白诱导人间充质干细胞定向分化为肌腱细胞，证明其与力学刺激协同促进肌腱样组织形成，为组织工程提供新策略。
3. **文献名称**：*Molecular characterization of Scleraxis-positive cells during tendon-to-bone insertion development*
**作者**：Shukunami C. et al.
**摘要**：通过重组SCXB蛋白的体外实验，揭示了其与TGF-β信号通路协同调控肌腱-骨界面分化的分子机制，强调了SCXB在组织再生中的潜在应用价值。
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**注**：SCXB通常被称为Scleraxis(简称SCX或Scx)，文献中可能存在命名差异。上述研究均涉及SCXB重组蛋白的功能验证或应用探索，适用于肌腱发育、干细胞分化及组织工程领域参考。
**Background of SCXB Recombinant Protein**
SCXB (Scleraxis Basic Helix-Loop-Helix Transcription Factor) is a key regulatory protein involved in the development and maintenance of tendons and ligaments. As a member of the basic helix-loop-helix (bHLH) transcription factor family, SCXB plays a critical role in differentiating mesenchymal stem cells into tenocytes and ligamentocytes, ensuring proper extracellular matrix (ECM) synthesis and tissue organization. It is predominantly expressed in connective tissues during embryogenesis and remains active in adult tendon repair and homeostasis.
The recombinant SCXB protein is engineered using biotechnological methods, such as *in vitro* expression systems (e.g., bacterial, mammalian, or insect cells), to produce a purified, functional form of the native protein. This allows researchers to study its molecular mechanisms, including its interaction with DNA-binding E-box motifs to activate tendon-specific genes like *Col1a1*, *Col3a1*, and *Tnmd*.
SCXB recombinant protein has gained attention in regenerative medicine, particularly for treating tendon injuries and degenerative disorders. Studies explore its potential to enhance tendon repair by promoting tenocyte proliferation, ECM remodeling, and reducing fibrosis. Additionally, it serves as a tool for *in vitro* modeling of tendon development and screening therapeutic compounds. Challenges remain in optimizing delivery methods and ensuring sustained bioactivity *in vivo*, but ongoing research underscores its promise in advancing musculoskeletal therapies.
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