| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SESN1 |
| Uniprot No | Q9Y6P5-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-551aa |
| 氨基酸序列 | AEGENEVRWDGLCSRDSTTRETALENIRQTILRKTEYLRSVKETPHRPSD GLSNTESSDGLNKLLAHLLMLSKRCPFKDVREKSEFILKSIQELGIRIPR PLGQGPSRFIPEKEILQVGSEDAQMHALFADSFAALGRLDNITLVMVFHP QYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVNDFL HVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEH SWSLAELVHAVVLLTHYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYC ICDITNGNHSVDEMPVNSAENVSVSDSFFEVEALMEKMRQLQECRDEEEA SQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRH GMHVPTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAM HKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTVVC TPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SESN1重组蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **文献名称**: "Recombinant SESN1 Protein Attenuates Oxidative Stress in Hepatocellular Carcinoma Cells"
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统表达并纯化了重组SESN1蛋白，发现其能显著抑制肝癌细胞中活性氧(ROS)的积累，并通过激活AMPK/mTOR通路抑制肿瘤细胞增殖。
2. **文献名称**: "Structural Characterization of Human Sestrin1 by X-ray Crystallography"
**作者**: Kim J, et al.
**摘要**: 利用重组SESN1蛋白解析了其晶体结构，揭示了其氧化还原敏感结构域的关键氨基酸位点，为研究SESN1在氧化应激中的分子机制提供了结构基础。
3. **文献名称**: "SESN1 Recombinant Protein Enhances Autophagy in Neurodegenerative Models"
**作者**: Chen R, et al.
**摘要**: 通过哺乳动物细胞表达系统获得高纯度SESN1重组蛋白，证明其在帕金森病模型中通过促进自噬清除异常蛋白聚集，具有神经保护作用。
4. **文献名称**: "Development of a SESN1-Based Therapeutic Protein for Metabolic Syndrome"
**作者**: Wang Y, et al.
**摘要**: 研究利用重组SESN1蛋白在肥胖小鼠模型中验证其调节脂代谢和胰岛素敏感性的功能，表明其作为代谢性疾病治疗药物的潜力。
**注意**：以上文献为示例，实际研究中建议通过 **PubMed** 或 **Google Scholar** 搜索关键词(如 "SESN1 recombinant protein")获取真实文献。
**Background of SESN1 Recombinant Protein**
SESN1 (Sestrin 1) is a stress-inducible protein belonging to the Sestrin family, which plays a critical role in cellular responses to oxidative and genotoxic stress. Initially identified as a transcriptional target of the tumor suppressor p53. SESN1 is involved in regulating antioxidant defenses, metabolic homeostasis, and aging-related pathways. It functions as a redox sensor, modulating the activity of key signaling pathways such as AMP-activated protein kinase (AMPK) and mechanistic target of rapamycin complex 1 (mTORC1), which are central to energy metabolism and cell growth.
The recombinant SESN1 protein is produced through genetic engineering, typically using bacterial or mammalian expression systems, to ensure high purity and biological activity. Its recombinant form retains the native protein’s ability to interact with cellular components, making it a valuable tool for studying stress response mechanisms, autophagy, and metabolic regulation. Researchers employ SESN1 recombinant protein in *in vitro* and *in vivo* models to investigate its role in diseases linked to oxidative stress, including cancer, neurodegenerative disorders, and metabolic syndromes.
Recent studies highlight SESN1’s potential therapeutic applications, particularly in mitigating age-related pathologies and enhancing stress resilience. Preclinical data suggest that SESN1 overexpression or supplementation may protect against DNA damage, improve mitochondrial function, and suppress tumor growth. However, challenges remain in optimizing delivery methods and understanding tissue-specific effects.
Overall, SESN1 recombinant protein serves as both a research reagent and a promising candidate for developing interventions targeting stress-related diseases, underscoring its importance in translational biomedicine.
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