| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKCB |
| Uniprot No | P05771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-671 aa |
| 活性数据 | ADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR DVLIVLVRDA KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL SVEIWDWDLT SRNDFMGSLS FGISELQKAS VDGWFKLLSQ EEGEYFNVPV PPEGSEANEE LRQKFERAKI SQGTKVPEEK TTNTVSKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM NLDQNEFAGF SYTNPEFVIN V |
| 分子量 | 76.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PRKCB蛋白的3篇示例文献及其摘要(注：文献为虚构示例，仅作格式参考)：
1. **文献名称**: "Recombinant Human PRKCB Expression and Its Role in Leukemia Cell Signaling"
**作者**: Zhang L. et al.
**摘要**: 本研究利用HEK293细胞系统成功表达并纯化重组人PRKCB蛋白，验证其在急性髓系白血病细胞中的促生存功能。实验表明，PRKCB通过磷酸化STAT3通路抑制癌细胞凋亡，提示其作为潜在治疗靶点。
2. **文献名称**: "Crystal Structure of PRKCB1 Kinase Domain Reveals ATP-Binding Mechanism"
**作者**: Tanaka K. et al.
**摘要**: 通过昆虫细胞表达系统获得重组人PRKCB1激酶结构域蛋白，解析其2.1Å晶体结构，发现独特的ATP结合口袋构象，为设计选择性抑制剂提供结构基础。
3. **文献名称**: "PRKCB-Mediated Tau Phosphorylation in Alzheimer's Disease Models"
**作者**: Gupta R. et al.
**摘要**: 使用大肠杆菌表达的重组PRKCB蛋白，证明其可在体外特异性磷酸化tau蛋白的Ser214位点。动物模型显示PRKCB抑制剂减少神经纤维缠结，提示与阿尔茨海默病病理相关。
(注：实际文献需通过PubMed/Google Scholar以"PRKCB recombinant protein"、"human PRKCB expression"等关键词检索。)
Recombinant human PRKCB (Protein Kinase C Beta) is a serine/threonine kinase belonging to the PKC family, which plays critical roles in signal transduction pathways regulating cell growth, differentiation, apoptosis, and immune responses. The PRKCB gene encodes two splice variants, PRKCB1 and PRKCB2. differing in their C-terminal sequences. Structurally, PRKCB contains a regulatory domain with tandem C1 domains for diacylglycerol (DAG) binding and a C2 domain for calcium sensitivity, followed by a catalytic kinase domain. Activation typically occurs via phospholipid hydrolysis, which releases DAG and elevates intracellular calcium, promoting membrane translocation and phosphorylation of downstream targets like NF-κB and MAPK cascades.
PRKCB is particularly notable in immune cell signaling, influencing B-cell development, T-cell activation, and inflammatory responses. Dysregulation of PRKCB is linked to pathologies, including cancer (enhancing tumor proliferation/metastasis), diabetic complications (via hyperglycemia-induced vascular damage), and neurological disorders. Recombinant PRKCB proteins, produced in expression systems like *E. coli* or mammalian cells, are essential tools for studying its biochemical properties, substrate interactions, and therapeutic targeting. Inhibitors targeting PRKCB’s ATP-binding or regulatory domains are under investigation for conditions like leukemia and solid tumors. Its dual role in pro-survival and apoptotic signaling underscores context-dependent complexity, driving ongoing research to unravel isoform-specific functions and therapeutic applications.
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